Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4213203..4213822 Replicon chromosome
Accession NZ_CP091058
Organism Klebsiella pneumoniae strain KP30086

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag L1063_RS20385 Protein ID WP_002892050.1
Coordinates 4213604..4213822 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag L1063_RS20380 Protein ID WP_002892066.1
Coordinates 4213203..4213577 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1063_RS20370 (4208355) 4208355..4209548 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L1063_RS20375 (4209571) 4209571..4212717 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
L1063_RS20380 (4213203) 4213203..4213577 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
L1063_RS20385 (4213604) 4213604..4213822 + 219 WP_002892050.1 HHA domain-containing protein Toxin
L1063_RS20390 (4213981) 4213981..4214547 + 567 WP_002892030.1 maltose O-acetyltransferase -
L1063_RS20395 (4214519) 4214519..4214659 - 141 WP_004147370.1 hypothetical protein -
L1063_RS20400 (4214680) 4214680..4215150 + 471 WP_002892026.1 YlaC family protein -
L1063_RS20405 (4215125) 4215125..4216576 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
L1063_RS20410 (4216677) 4216677..4217375 + 699 WP_002892021.1 GNAT family protein -
L1063_RS20415 (4217372) 4217372..4217512 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
L1063_RS20420 (4217512) 4217512..4217775 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T232581 WP_002892050.1 NZ_CP091058:4213604-4213822 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T232581 NZ_CP127389:c3689570-3689277 [Proteus sp. HZ0627]
ATGATCTTTAATATTGAGTTTGATAAGCGTGCATTAAAAGAATGGCAGAAACTTGATCAAAGCATTAAAGAACAATTTAA
AAAGAAATTAAAAAAATTACAGGAAAACCCTTATATAGAGTCTGCTCGCTTAAAAGGTGATCTCTCGGGTTGCTATAAGA
TTAAATTACGAGCATCTGGTTTTCGATTGGTTTACCAAATCATTGATTCAGAAGTCGTTATTTTAGTTATTGCTATTGGT
AAGCGTGAAGAAAGTAAAGCGTATTCATTAGCTGAAATGAGAATTCAAAAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT232581 WP_002892066.1 NZ_CP091058:4213203-4213577 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT232581 NZ_CP127389:c3689814-3689560 [Proteus sp. HZ0627]
ATGACAACAATACAAGTTAGAGTAGACGATGAGCTAAAAAAAGAAGCTTATCAAGTATTTGAAAAAATGAATCTATCTCC
TTCAGATGCTATAAGATTGTTTCTACGTTATGTTTCTGAAAATGAAAAGTTACCTTTCTCTGAAGTTTCTGTCGTTGTTG
GTGAACAAGACGAAGATGAGGATATTTTAGCTGTTGTACGTGAGCGTTTGAAGAAACCATGTAAACGTATAAGAGTAAAT
ATTGATGATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References