Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 133779..134689 Replicon plasmid pSW25HRG
Accession NZ_CP091050
Organism Klebsiella pneumoniae strain AHSWKP25

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag L1P15_RS27485 Protein ID WP_004144067.1
Coordinates 133779..134249 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag L1P15_RS27490 Protein ID WP_023287184.1
Coordinates 134246..134689 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P15_RS27460 (L1P15_27460) 129853..130821 - 969 WP_241556116.1 IS5 family transposase -
L1P15_RS27465 (L1P15_27465) 130812..130994 + 183 Protein_139 IS481 family transposase -
L1P15_RS27470 (L1P15_27470) 130998..131771 + 774 Protein_140 integrase core domain-containing protein -
L1P15_RS27475 (L1P15_27475) 132344..132604 + 261 WP_017901409.1 hypothetical protein -
L1P15_RS27480 (L1P15_27480) 132724..133692 - 969 WP_000654811.1 IS5 family transposase -
L1P15_RS27485 (L1P15_27485) 133779..134249 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
L1P15_RS27490 (L1P15_27490) 134246..134689 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
L1P15_RS27495 (L1P15_27495) 134790..134873 - 84 Protein_145 DUF4113 domain-containing protein -
L1P15_RS27505 (L1P15_27505) 135645..135740 + 96 Protein_147 integrase core domain-containing protein -
L1P15_RS27510 (L1P15_27510) 135724..136355 + 632 Protein_148 DUF4113 domain-containing protein -
L1P15_RS27515 (L1P15_27515) 136434..136662 + 229 Protein_149 type II toxin-antitoxin system Phd/YefM family antitoxin -
L1P15_RS27520 (L1P15_27520) 137513..138484 - 972 WP_023287152.1 ParB/RepB/Spo0J family plasmid partition protein -
L1P15_RS27525 (L1P15_27525) 138484..139650 - 1167 WP_023287153.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..140379 140379
- inside IScluster/Tn - - 129853..135393 5540


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T232567 WP_004144067.1 NZ_CP091050:c134249-133779 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T232567 NZ_CP127378:3990638-3990790 [Xanthomonas fragariae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGTAACACCTTTGCTGGCGC
CGGCAAGGATATGCAACGCGCGGGCAGCAAGGTCGAAAAGAAGGCCGATAACTGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT232567 WP_023287184.1 NZ_CP091050:c134689-134246 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT232567 NZ_CP127378:3990870-3991004 [Xanthomonas fragariae]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATACTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References