Detailed information of TA system TA232566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 76860..77611
• Replicon: plasmid pSW25HRG
• Accession: NZ_CP091050
• Organism: Klebsiella pneumoniae strain AHSWKP25

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: L1P15_RS27180
• Protein ID: WP_004902249.1
• Coordinates: 76860..77342 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: L1P15_RS27185
• Protein ID: WP_004902250.1
• Coordinates: 77333..77611 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P15_RS27160 (L1P15_27160)	73260..73907	-	648	WP_014386537.1	EcsC family protein	-
L1P15_RS27165 (L1P15_27165)	73934..74689	-	756	WP_117257231.1	DUF2971 domain-containing protein	-
L1P15_RS27170 (L1P15_27170)	74825..75181	-	357	WP_142915927.1	hypothetical protein	-
L1P15_RS27175 (L1P15_27175)	75286..75825	-	540	WP_004902239.1	hypothetical protein	-
L1P15_RS27180 (L1P15_27180)	76860..77342	-	483	WP_004902249.1	GNAT family N-acetyltransferase	Toxin
L1P15_RS27185 (L1P15_27185)	77333..77611	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
L1P15_RS27190 (L1P15_27190)	77730..77942	-	213	WP_004902255.1	hypothetical protein	-
L1P15_RS27195 (L1P15_27195)	78050..78391	-	342	WP_004902257.1	hypothetical protein	-
L1P15_RS27200 (L1P15_27200)	79298..79612	+	315	WP_023288299.1	hypothetical protein	-
L1P15_RS27205 (L1P15_27205)	79719..80008	-	290	Protein_87	Tn3 family transposase	-
L1P15_RS27210 (L1P15_27210)	80059..80811	-	753	WP_004729622.1	alpha/beta hydrolase	-
L1P15_RS27215 (L1P15_27215)	81052..81921	+	870	WP_001567390.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..140379	140379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17689.52 Da
• Isoelectric Point: 9.2033

>T232566 WP_004902249.1 NZ_CP091050:c77342-76860 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNKVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T232566 NZ_CP127378:2069473-2069892 [Xanthomonas fragariae]
ATGATTGTCTTGGATACGAATGTAGTCTCCGAGGCGATAAAACCCGAACCCAACCCGGCCGTGCGAGCTTGGTTGAATGA
GCAGGTAGCGGAAACCCTCTACCTGTCCAGCGTGACGCTTGCCGAACTGCTGTTCGGCATCGGCGCGCTGCCGAACGGGA
AACGTAAGAAAGGCCTGGGCGAAGCACTGGACGGCTTGCTCGAACTGTTCGGCGAGCGGGTGCTGATGTTCGACACCGAA
GCGGCTCGCCATTATGCCGAGCTGGCGGTGAAGGCCCGCACAGCCGGGAAGGGTTTTCCAACACCTGACGGATATATTGG
GGCTATTGCGGCCTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTCGAAGCGGCTGGGCTTACTGTTATCA
ATCCTTGGAACCATCAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT232566 WP_004902250.1 NZ_CP091050:c77611-77333 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT232566 NZ_CP127378:2069222-2069476 [Xanthomonas fragariae]
ATGGCAATCCTGACAGTGCGGAATGTGCCCGACGAGGTGCATCGCGCTCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTCCGCGAGATTCTGGAGAGTGCAGTTAAGTCAGAAAAGCGCATCCGCATGGGCGACGCGTTGGCGG
AGCTTGGCCGCCAAGTAGTGCTGACAAACGATGATTTCGCAGTGTTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCG
ATGAGGTTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3