232564

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5166780..5167405 Replicon chromosome
Accession NZ_CP091048
Organism Klebsiella pneumoniae strain AHSWKP25

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag L1P15_RS25080 Protein ID WP_002882817.1
Coordinates 5166780..5167163 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L1P15_RS25085 Protein ID WP_004150355.1
Coordinates 5167163..5167405 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P15_RS25065 (5164146) 5164146..5165048 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L1P15_RS25070 (5165045) 5165045..5165680 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L1P15_RS25075 (5165677) 5165677..5166606 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L1P15_RS25080 (5166780) 5166780..5167163 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
L1P15_RS25085 (5167163) 5167163..5167405 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L1P15_RS25090 (5167610) 5167610..5168527 + 918 WP_015959199.1 alpha/beta hydrolase -
L1P15_RS25095 (5168541) 5168541..5169482 - 942 WP_153932582.1 fatty acid biosynthesis protein FabY -
L1P15_RS25100 (5169527) 5169527..5169964 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L1P15_RS25105 (5169961) 5169961..5170821 - 861 WP_004146232.1 virulence factor BrkB family protein -
L1P15_RS25110 (5170815) 5170815..5171414 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T232564 WP_002882817.1 NZ_CP091048:c5167163-5166780 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T232564 NZ_CP127376:243148-243498 [Bacillus arachidis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGGAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAGAAAG
CAAAATTGCCCACGCATGTGGAAATTGATGCGAAAAAGTACGGGTTTGAGAGGGATTCTGTTATCTTGCTCGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAGGTGATGATGAGTCGTGTAGATGAAGCTTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT232564 WP_004150355.1 NZ_CP091048:c5167405-5167163 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT232564 NZ_CP127376:242856-243143 [Bacillus arachidis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCGAAGCAAGTGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References