Detailed information of TA system TA232561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4561744..4562554
• Replicon: chromosome
• Accession: NZ_CP091048
• Organism: Klebsiella pneumoniae strain AHSWKP25

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A060VJ83
• Locus tag: L1P15_RS22235
• Protein ID: WP_004178461.1
• Coordinates: 4561744..4562277 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A483I2M4
• Locus tag: L1P15_RS22240
• Protein ID: WP_004222378.1
• Coordinates: 4562288..4562554 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P15_RS22230 (4560575)	4560575..4561696	+	1122	WP_002887282.1	cupin domain-containing protein	-
L1P15_RS22235 (4561744)	4561744..4562277	-	534	WP_004178461.1	type II toxin-antitoxin system toxin KacT	Toxin
L1P15_RS22240 (4562288)	4562288..4562554	-	267	WP_004222378.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
L1P15_RS22245 (4562657)	4562657..4564090	-	1434	WP_004222374.1	Cu(+)/Ag(+) sensor histidine kinase	-
L1P15_RS22250 (4564080)	4564080..4564763	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
L1P15_RS22255 (4564935)	4564935..4566320	+	1386	WP_004222371.1	efflux transporter outer membrane subunit	-
L1P15_RS22260 (4566338)	4566338..4566682	+	345	WP_004222368.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19810.65 Da
• Isoelectric Point: 5.2614

>T232561 WP_004178461.1 NZ_CP091048:c4562277-4561744 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T232561 NZ_CP127363:2934767-2935690 [Paracidovorax citrulli]
TTGGCCCATCCTCAAAACTGGCGCCCCGAGCGGCGTCAATTGCATGACCGGCTGATCGGCGATGCGAAAACTGCAGCCCA
GGATTTCGCCGAGGTGATCGAGCGCGGTGGCCACCCACCGACATTGTTCGCGTTGCGAGGCAATACGGCTGCGGGAAAGA
CACGCATGGCGACACAAACCATCCCCGTACTCGCCAATGCCCTCAAGGAGAGTGGCGGCGCCGGCTGTATTAATCCCGAC
ATCTTCAAACGTTCGCTCGCAGAAACTCCAGAGGGAGCAAAACTTACCTCGGCGCAGGTACACGCCGAGTCGTGCATCCT
CGCTGACCGGCTTGAAGACGAACTCCGATTGCAGAAGACCGCGAGCGGCGCGGCGATCAGCATGCTGGTCGATAAGCGAC
TGGCTGGAGCGCACGAAATCGACGCGTACATCAAGCTGGCTCAGGAGACCGGTCGCAAAATCGAGTTGTGCGATATTGAT
GCTCCACTCGAGCAGTCACTCATGGGCGTGCTGCAGCGCAAGCCCGAAGGTGACGCCCCCCGGCCACCCTACGTTGCGGT
TGCCAACGGATTCTCGGCGGTTCGCGGGCACCGGCTCGATGTCATCGACAAGTTTCTCTCGAACCCGACCTTGGGGAGCT
ATCATCTATTCGGCACTACCGGGAATGGCAGCAAGGCGATGGTTGCCAGCGTCGTCAACGGCGAACTGAGCATTCATGAC
CCTCAGTTATACGAGGCCATTACAGATCCCCAAGGTGCCCGGGCAGGCGATAGCGGCGATCAGATCATTGACGCTGCGCT
GATCGACCGGCTGACCAAAGGTATAGACGACCCAGCGCGAGCCATGGATGCCCGCACCGCATTGGAGCGGTACGCGGGCA
TGACGTGGTCGGACGCTCTGGCCGCCCATAGTTCACTGACTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9969.44 Da
• Isoelectric Point: 10.1757

>AT232561 WP_004222378.1 NZ_CP091048:c4562554-4562288 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT232561 NZ_CP127363:2935705-2936001 [Paracidovorax citrulli]
ATGACAGGCCTGAAAGGCGCAGATGCGCTCGACTTCCATAAGAAACTCAAGGAACGCAATGCTGCTTTACGCAATGCGGA
GTTGAACGCGGCGAAGGAACTGGCCGACAAAAATGGCAAGGAGCCTTTTGATCTCGTGAAACTGGAGAGGCTTTGCGATA
CCAGTCAAGCAGGCCGCCTTGTTGAAGGGACCGAACGGCAAGCCATGTACGAATACATGTACTACGTCGAGTACAAGAAT
GTTGGCACGTTGCAGGATTTCGCCCGGGTGGTGACAACGCTCGCGTCCTGGTCTTAA

Structures

Toxin

• AlphaFold DB: A0A060VJ83

Antitoxin

• AlphaFold DB: A0A483I2M4