Detailed information of TA system TA232545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 54934..55559
• Replicon: plasmid p3_STEC346
• Accession: NZ_CP091047
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L1P11_RS26640
• Protein ID: WP_000911308.1
• Coordinates: 54934..55332 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8S7CUH2
• Locus tag: L1P11_RS26645
• Protein ID: WP_000450522.1
• Coordinates: 55332..55559 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS26620 (52133)	52133..52780	+	648	WP_000015959.1	hypothetical protein	-
L1P11_RS26625 (52852)	52852..53589	+	738	WP_000926073.1	conjugal transfer complement resistance protein TraT	-
L1P11_RS26630 (53717)	53717..54409	+	693	WP_000235172.1	hypothetical protein	-
L1P11_RS26635 (54551)	54551..54919	+	369	WP_235412088.1	DUF2726 domain-containing protein	-
L1P11_RS26640 (54934)	54934..55332	-	399	WP_000911308.1	type II toxin-antitoxin system VapC family toxin	Toxin
L1P11_RS26645 (55332)	55332..55559	-	228	WP_000450522.1	toxin-antitoxin system antitoxin VapB	Antitoxin
L1P11_RS26650 (55692)	55692..57878	+	2187	WP_000099429.1	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / tet(A)	-	1..82263	82263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14972.34 Da
• Isoelectric Point: 8.1432

>T232545 WP_000911308.1 NZ_CP091047:c55332-54934 [Escherichia coli]
MLKFMLDTNICIFTIKNKPAHIRERFNLNTGRMCISSVTVMELVYGAEKSQFPERNLAIIEGFISRLDVLDYDCAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERIAGLRLEDWC

Nucleotide

• Length: 399 bp

>T232545 NZ_CP127316:4171951-4172328 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8368.36 Da
• Isoelectric Point: 4.3569

>AT232545 WP_000450522.1 NZ_CP091047:c55559-55332 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVDVIAVGRTRIITPAGESWDSWFDGDSVSADFMNDREQPAVQERDAF

Nucleotide

• Length: 228 bp

>AT232545 NZ_CP127316:4171493-4171861 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGTGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA