Detailed information of TA system TA232541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 76657..77204
• Replicon: plasmid p2_STEC346
• Accession: NZ_CP091046
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: L1P11_RS26240
• Protein ID: WP_248699117.1
• Coordinates: 76926..77204 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: L1P11_RS26235
• Protein ID: WP_000079941.1
• Coordinates: 76657..76926 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS26200 (71718)	71718..71921	+	204	WP_001327131.1	hypothetical protein	-
L1P11_RS26205 (72664)	72664..73124	+	461	Protein_96	thermonuclease family protein	-
L1P11_RS26860 (73475)	73475..73609	+	135	WP_001058542.1	hypothetical protein	-
- (73784)	73784..73845	-	62	NuclAT_1	-	-
- (73784)	73784..73845	-	62	NuclAT_1	-	-
- (73784)	73784..73845	-	62	NuclAT_1	-	-
- (73784)	73784..73845	-	62	NuclAT_1	-	-
L1P11_RS26210 (73889)	73889..74038	+	150	WP_001300273.1	Hok/Gef family protein	-
L1P11_RS26215 (74322)	74322..74570	+	249	WP_000083838.1	replication regulatory protein RepA	-
L1P11_RS26220 (74643)	74643..74768	-	126	WP_071590472.1	replication protein RepA	-
L1P11_RS26225 (74817)	74817..74891	+	75	WP_001365705.1	RepA leader peptide Tap	-
L1P11_RS26230 (74884)	74884..75741	+	858	WP_001775129.1	incFII family plasmid replication initiator RepA	-
L1P11_RS26235 (76657)	76657..76926	+	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
L1P11_RS26240 (76926)	76926..77204	+	279	WP_248699117.1	type II toxin-antitoxin system toxin YacB	Toxin
L1P11_RS26245 (77250)	77250..77369	+	120	Protein_105	3'-5' exonuclease	-
L1P11_RS26250 (77457)	77457..78395	+	939	Protein_106	IS21-like element IS21 family transposase	-
L1P11_RS26255 (78393)	78393..78554	-	162	Protein_107	IS66 family transposase	-
L1P11_RS26260 (78697)	78697..79133	-	437	WP_235412075.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..79133	79133
	-	flank	IS/Tn	-	-	77457..78455	998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10789.40 Da
• Isoelectric Point: 8.4615

>T232541 WP_248699117.1 NZ_CP091046:76926-77204 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDNQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T232541 NZ_CP127316:3912856-3913029 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT232541 WP_000079941.1 NZ_CP091046:76657-76926 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT232541 NZ_CP127316:3912532-3912828 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5