Detailed information of TA system TA232534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 8956..9503
• Replicon: plasmid p1_STEC346
• Accession: NZ_CP091045
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: L1P11_RS25370
• Protein ID: WP_247173439.1
• Coordinates: 9225..9503 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A3Z6J955
• Locus tag: L1P11_RS25365
• Protein ID: WP_071940833.1
• Coordinates: 8956..9225 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS25325 (4442)	4442..4633	+	192	WP_071849536.1	hypothetical protein	-
L1P11_RS25330 (4878)	4878..5339	+	462	WP_069723215.1	thermonuclease family protein	-
L1P11_RS25335 (5385)	5385..5594	+	210	WP_171935555.1	hemolysin expression modulator Hha	-
L1P11_RS25340 (5632)	5632..6222	+	591	WP_000766807.1	DUF2726 domain-containing protein	-
L1P11_RS25345 (6462)	6462..6722	+	261	WP_000078109.1	replication regulatory protein RepA	-
L1P11_RS25350 (6957)	6957..7031	+	75	WP_001365705.1	RepA leader peptide Tap	-
L1P11_RS25355 (7024)	7024..7881	+	858	WP_123005415.1	incFII family plasmid replication initiator RepA	-
L1P11_RS25360 (8743)	8743..8901	+	159	WP_235412045.1	hypothetical protein	-
L1P11_RS25365 (8956)	8956..9225	+	270	WP_071940833.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
L1P11_RS25370 (9225)	9225..9503	+	279	WP_247173439.1	type II toxin-antitoxin system toxin YacB	Toxin
L1P11_RS25375 (9610)	9610..10773	+	1164	WP_140431710.1	hypothetical protein	-
L1P11_RS25380 (11038)	11038..11397	-	360	WP_001559870.1	YcgJ family protein	-
L1P11_RS25385 (11497)	11497..11691	-	195	WP_235412047.1	hypothetical protein	-
L1P11_RS25390 (12331)	12331..12510	-	180	WP_001559872.1	hypothetical protein	-
L1P11_RS25395 (12851)	12851..13831	+	981	WP_001559873.1	plasmid segregation protein ParM	-
L1P11_RS25400 (13834)	13834..14256	+	423	WP_000774832.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..63171	63171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10774.39 Da
• Isoelectric Point: 8.4614

>T232534 WP_247173439.1 NZ_CP091045:9225-9503 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASGLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQREKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T232534 NZ_CP127316:c2244306-2243971 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10311.69 Da
• Isoelectric Point: 10.1734

>AT232534 WP_071940833.1 NZ_CP091045:8956-9225 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLKRGDVLPHDMVESSATAWRD
EMSRKVTGK

Nucleotide

• Length: 270 bp

>AT232534 NZ_CP127316:c2244554-2244306 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3Z6J955