Detailed information of TA system TA232532

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4442135..4442969
• Replicon: chromosome
• Accession: NZ_CP091044
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: L1P11_RS22160
• Protein ID: WP_000854770.1
• Coordinates: 4442135..4442512 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: L1P11_RS22165
• Protein ID: WP_001332042.1
• Coordinates: 4442601..4442969 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS22135 (4438311)	4438311..4439933	-	1623	WP_001295726.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-
L1P11_RS22140 (4440659)	4440659..4440835	-	177	Protein_4336	helix-turn-helix domain-containing protein	-
L1P11_RS22145 (4441202)	4441202..4441351	-	150	Protein_4337	hypothetical protein	-
L1P11_RS22150 (4441457)	4441457..4441633	-	177	WP_000839286.1	DUF957 domain-containing protein	-
L1P11_RS22155 (4441650)	4441650..4442138	-	489	WP_000761690.1	DUF5983 family protein	-
L1P11_RS22160 (4442135)	4442135..4442512	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
L1P11_RS22165 (4442601)	4442601..4442969	-	369	WP_001332042.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L1P11_RS22170 (4443132)	4443132..4443353	-	222	WP_000692323.1	DUF987 domain-containing protein	-
L1P11_RS22175 (4443416)	4443416..4443892	-	477	WP_001186779.1	RadC family protein	-
L1P11_RS22180 (4443908)	4443908..4444372	-	465	WP_000855061.1	antirestriction protein	-
L1P11_RS22185 (4444714)	4444714..4445532	-	819	WP_235411689.1	DUF932 domain-containing protein	-
L1P11_RS22190 (4445650)	4445650..4445845	-	196	Protein_4346	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4437128..4475701	38573
	-	flank	IS/Tn	-	-	4440246..4440395	149

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T232532 WP_000854770.1 NZ_CP091044:c4442512-4442135 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T232532 NZ_CP127316:c1186373-1186270 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13677.38 Da
• Isoelectric Point: 6.5931

>AT232532 WP_001332042.1 NZ_CP091044:c4442969-4442601 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT232532 NZ_CP127316:1186232-1186500 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCTG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9