Detailed information of TA system TA232518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1438224..1438849
• Replicon: chromosome
• Accession: NZ_CP091044
• Organism: Escherichia coli strain STEC346

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L1P11_RS07155
• Protein ID: WP_000911330.1
• Coordinates: 1438451..1438849 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: L1P11_RS07150
• Protein ID: WP_000450524.1
• Coordinates: 1438224..1438451 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P11_RS07125 (1434027)	1434027..1434497	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
L1P11_RS07130 (1434497)	1434497..1435069	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
L1P11_RS07135 (1435215)	1435215..1436093	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
L1P11_RS07140 (1436110)	1436110..1437144	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
L1P11_RS07145 (1437357)	1437357..1438070	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
L1P11_RS07150 (1438224)	1438224..1438451	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
L1P11_RS07155 (1438451)	1438451..1438849	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
L1P11_RS07160 (1438996)	1438996..1439859	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
L1P11_RS07165 (1439874)	1439874..1441889	+	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
L1P11_RS07170 (1441963)	1441963..1442661	+	699	WP_000679812.1	esterase	-
L1P11_RS07175 (1442771)	1442771..1442971	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T232518 WP_000911330.1 NZ_CP091044:1438451-1438849 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T232518 NZ_CP127314:c4640777-4640463 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT232518 WP_000450524.1 NZ_CP091044:1438224-1438451 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT232518 NZ_CP127314:c4641013-4640780 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33