Detailed information of TA system TA232508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 840258..840819
• Replicon: chromosome
• Accession: NZ_CP091032
• Organism: Mycoplasma feriruminatoris strain G5847

Toxin (Protein)

• Gene name: relE
• Uniprot ID: L5LAX5
• Locus tag: D500_RS03605
• Protein ID: WP_008362621.1
• Coordinates: 840258..840554 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L5LA09
• Locus tag: D500_RS03610
• Protein ID: WP_008362623.1
• Coordinates: 840547..840819 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D500_RS03585 (D500_00724)	835369..835998	+	630	WP_008362614.1	beta-phosphoglucomutase	-
D500_RS03590 (D500_00725)	836106..837770	+	1665	WP_008362615.1	LppA family lipoprotein	-
D500_RS03595 (D500_00726)	837798..838679	-	882	WP_008362617.1	Cof-type HAD-IIB family hydrolase	-
D500_RS03600 (D500_00727)	838928..840223	+	1296	WP_008362619.1	ATP-binding protein	-
D500_RS03605 (D500_00728)	840258..840554	-	297	WP_008362621.1	type II toxin-antitoxin system YafQ family toxin	Toxin
D500_RS03610 (D500_00729)	840547..840819	-	273	WP_008362623.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
D500_RS03615 (D500_00730)	841004..842173	-	1170	WP_008362626.1	site-specific DNA-methyltransferase	-
D500_RS03620 (D500_00731)	842337..845441	-	3105	WP_008362628.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-87)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11380.32 Da
• Isoelectric Point: 9.9386

>T232508 WP_008362621.1 NZ_CP091032:c840554-840258 [Mycoplasma feriruminatoris]
MTKYKIVTTKFKKDLKLAKKQNKDLNKLDYVINILTQGKELDAKYQDHSLTGIYKGYRECHIDPDLLIYKKDDDKIVIIM
LRLGSHSNLFEKQKNY

Nucleotide

• Length: 297 bp

>T232508 NZ_CP127314:2317848-2318102 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10257.67 Da
• Isoelectric Point: 4.8483

>AT232508 WP_008362623.1 NZ_CP091032:c840819-840547 [Mycoplasma feriruminatoris]
MKTTNLNVRIDKEVKHDAEAIFNELGLSTSAAINMFFKATIRNNSLPFSTKLDTPNKETLLAMKEAEELLKDPNTKYYSS
FKELLETLDD

Nucleotide

• Length: 273 bp

>AT232508 NZ_CP127314:2317600-2317851 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: L5LAX5

Antitoxin

• AlphaFold DB: L5LA09