Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 204459..206082 Replicon plasmid p1_STEC384
Accession NZ_CP091030
Organism Escherichia coli strain STEC384

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag L1P14_RS25500 Protein ID WP_000088046.1
Coordinates 204459..205784 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag L1P14_RS25505 Protein ID WP_024136327.1
Coordinates 205789..206082 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P14_RS25470 200443..201300 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
L1P14_RS25475 201316..201624 - 309 WP_001224687.1 hypothetical protein -
L1P14_RS25480 202173..202598 - 426 WP_001043843.1 hypothetical protein -
L1P14_RS25485 202852..203667 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
L1P14_RS25490 203680..204090 - 411 WP_000985911.1 hypothetical protein -
L1P14_RS25495 204192..204398 - 207 WP_000134171.1 hypothetical protein -
L1P14_RS25500 204459..205784 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
L1P14_RS25505 205789..206082 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
L1P14_RS25510 206686..206868 - 183 WP_235418679.1 hypothetical protein -
L1P14_RS25515 206917..207714 - 798 WP_000544828.1 IS21-like element IS21 family helper ATPase IstB -
L1P14_RS25520 207714..208886 - 1173 WP_015056461.1 IS21-like element IS21 family transposase -
L1P14_RS25525 208968..209213 - 246 WP_235418681.1 hypothetical protein -
L1P14_RS25530 209586..209927 + 342 WP_001151575.1 hypothetical protein -
L1P14_RS25535 210019..210210 + 192 WP_000398480.1 hypothetical protein -
L1P14_RS25540 210260..210541 + 282 WP_000077926.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid oqxA / oqxB / tet(A) / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia - 1..229883 229883


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T232503 WP_000088046.1 NZ_CP091030:c205784-204459 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T232503 NZ_CP127314:c980663-980199 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT232503 WP_024136327.1 NZ_CP091030:c206082-205789 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT232503 NZ_CP127314:c980998-980663 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References