Detailed information of TA system TA232498

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3894546..3895374
• Replicon: chromosome
• Accession: NZ_CP091029
• Organism: Escherichia coli strain STEC384

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: L1P14_RS19310
• Protein ID: WP_235399408.1
• Coordinates: 3894546..3894923 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: L1P14_RS19315
• Protein ID: WP_235399409.1
• Coordinates: 3895006..3895374 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P14_RS19280 (3890130)	3890130..3891449	+	1320	WP_000144688.1	site-specific integrase	-
L1P14_RS19285 (3891542)	3891542..3891829	-	288	Protein_3781	DUF4942 domain-containing protein	-
L1P14_RS19290 (3892111)	3892111..3893115	-	1005	WP_001179707.1	IS110-like element ISSfl8 family transposase	-
L1P14_RS19295 (3893187)	3893187..3893747	-	561	Protein_3783	DUF4942 domain-containing protein	-
L1P14_RS19300 (3893844)	3893844..3894041	-	198	WP_000772024.1	DUF957 domain-containing protein	-
L1P14_RS19305 (3894061)	3894061..3894549	-	489	WP_032215286.1	DUF5983 family protein	-
L1P14_RS19310 (3894546)	3894546..3894923	-	378	WP_235399408.1	TA system toxin CbtA family protein	Toxin
L1P14_RS19315 (3895006)	3895006..3895374	-	369	WP_235399409.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L1P14_RS19320 (3895537)	3895537..3895758	-	222	WP_137446885.1	DUF987 domain-containing protein	-
L1P14_RS19325 (3895821)	3895821..3896297	-	477	WP_001186747.1	RadC family protein	-
L1P14_RS19330 (3896313)	3896313..3896786	-	474	WP_001696846.1	antirestriction protein	-
L1P14_RS19335 (3896880)	3896880..3897125	-	246	WP_000680586.1	hypothetical protein	-
L1P14_RS19340 (3897125)	3897125..3897943	-	819	WP_089581163.1	DUF932 domain-containing protein	-
L1P14_RS19345 (3898283)	3898283..3899353	-	1071	WP_109955363.1	patatin-like phospholipase family protein	-
L1P14_RS19350 (3899350)	3899350..3900255	-	906	WP_001696852.1	diguanylate cyclase regulator RdcB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3892111..3893115	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14305.36 Da
• Isoelectric Point: 8.7198

>T232498 WP_235399408.1 NZ_CP091029:c3894923-3894546 [Escherichia coli]
MKTLPDTHVRKASRCPSPVTVWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T232498 NZ_CP127308:c57573-57193 [Escherichia coli]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.47 Da
• Isoelectric Point: 6.7392

>AT232498 WP_235399409.1 NZ_CP091029:c3895374-3895006 [Escherichia coli]
VSDKLSGITHPDDNHDRSWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT232498 NZ_CP127308:c57794-57573 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA