Detailed information of TA system TA232497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3730477..3731314
• Replicon: chromosome
• Accession: NZ_CP091029
• Organism: Escherichia coli strain STEC384

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: L1P14_RS18555
• Protein ID: WP_000227784.1
• Coordinates: 3730772..3731314 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: L1P14_RS18550
• Protein ID: WP_001297137.1
• Coordinates: 3730477..3730788 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P14_RS18525 (3725497)	3725497..3726444	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
L1P14_RS18530 (3726466)	3726466..3728457	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
L1P14_RS18535 (3728447)	3728447..3729061	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
L1P14_RS18540 (3729061)	3729061..3729390	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
L1P14_RS18545 (3729402)	3729402..3730292	+	891	WP_000971336.1	heme o synthase	-
L1P14_RS18550 (3730477)	3730477..3730788	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
L1P14_RS18555 (3730772)	3730772..3731314	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
L1P14_RS18560 (3731370)	3731370..3732305	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
L1P14_RS18565 (3732713)	3732713..3734077	+	1365	WP_001000975.1	MFS transporter	-
L1P14_RS18570 (3734205)	3734205..3734696	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
L1P14_RS18575 (3734864)	3734864..3735775	+	912	WP_000705877.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T232497 WP_000227784.1 NZ_CP091029:3730772-3731314 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T232497 NZ_CP127306:c4342992-4342615 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT232497 WP_001297137.1 NZ_CP091029:3730477-3730788 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT232497 NZ_CP127306:c4343450-4343082 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCAATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCTAACCGCGCAGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAAATCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGCTG
TGGTTGCGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3