23249

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1224593..1225377 Replicon chromosome
Accession NC_011601
Organism Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag E2348C_RS06265 Protein ID WP_000613626.1
Coordinates 1224593..1225087 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag E2348C_RS06270 Protein ID WP_001110446.1
Coordinates 1225084..1225377 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2348C_RS06245 (E2348_C_1170) 1219786..1220883 + 1098 WP_000589300.1 flagellar basal body P-ring protein FlgI -
E2348C_RS06250 (E2348_C_1171) 1220883..1221824 + 942 WP_012578881.1 flagellar assembly peptidoglycan hydrolase FlgJ -
E2348C_RS06255 (E2348_C_1172) 1221890..1223533 + 1644 WP_000096508.1 flagellar hook-associated protein FlgK -
E2348C_RS06260 (E2348_C_1173) 1223545..1224498 + 954 WP_001212764.1 flagellar hook-associated protein FlgL -
E2348C_RS06265 (E2348_C_1174) 1224593..1225087 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
E2348C_RS06270 (E2348_C_1175) 1225084..1225377 - 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
E2348C_RS06275 (E2348_C_1176) 1225511..1228696 - 3186 WP_000827393.1 ribonuclease E -
E2348C_RS06285 (E2348_C_1178) 1229269..1230228 + 960 WP_000846329.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T23249 WP_000613626.1 NC_011601:c1225087-1224593 [Escherichia coli O127:H6 str. E2348/69]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T23249 NC_011601:c1225087-1224593 [Escherichia coli O127:H6 str. E2348/69]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGTATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT23249 WP_001110446.1 NC_011601:c1225377-1225084 [Escherichia coli O127:H6 str. E2348/69]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT23249 NC_011601:c1225377-1225084 [Escherichia coli O127:H6 str. E2348/69]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References