Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1332180..1332805 Replicon chromosome
Accession NZ_CP091029
Organism Escherichia coli strain STEC384

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag L1P14_RS06485 Protein ID WP_000911329.1
Coordinates 1332407..1332805 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag L1P14_RS06480 Protein ID WP_000450524.1
Coordinates 1332180..1332407 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P14_RS06455 (1327982) 1327982..1328452 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
L1P14_RS06460 (1328452) 1328452..1329024 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
L1P14_RS06465 (1329170) 1329170..1330048 + 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
L1P14_RS06470 (1330065) 1330065..1331099 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
L1P14_RS06475 (1331312) 1331312..1332025 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
L1P14_RS06480 (1332180) 1332180..1332407 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
L1P14_RS06485 (1332407) 1332407..1332805 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
L1P14_RS06490 (1332952) 1332952..1333815 + 864 WP_001267498.1 neutral zinc metallopeptidase -
L1P14_RS06495 (1333830) 1333830..1335845 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
L1P14_RS06500 (1335919) 1335919..1336617 + 699 WP_000679823.1 esterase -
L1P14_RS06505 (1336727) 1336727..1336927 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T232484 WP_000911329.1 NZ_CP091029:1332407-1332805 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T232484 NZ_CP127306:2385543-2385950 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT232484 WP_000450524.1 NZ_CP091029:1332180-1332407 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT232484 NZ_CP127306:2385296-2385562 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References