Detailed information of TA system TA232481

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 789015..789850
• Replicon: chromosome
• Accession: NZ_CP091029
• Organism: Escherichia coli strain STEC384

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: L1P14_RS03845
• Protein ID: WP_032286705.1
• Coordinates: 789015..789392 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: L1P14_RS03850
• Protein ID: WP_235399448.1
• Coordinates: 789482..789850 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P14_RS03820 (784907)	784907..785443	+	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
L1P14_RS03825 (785778)	785778..787313	-	1536	WP_032286710.1	EAL domain-containing protein	-
L1P14_RS03830 (787384)	787384..788232	-	849	Protein_752	DUF4942 domain-containing protein	-
L1P14_RS03835 (788321)	788321..788518	-	198	WP_000839243.1	DUF957 domain-containing protein	-
L1P14_RS03840 (788530)	788530..789018	-	489	WP_235399447.1	DUF5983 family protein	-
L1P14_RS03845 (789015)	789015..789392	-	378	WP_032286705.1	TA system toxin CbtA family protein	Toxin
L1P14_RS03850 (789482)	789482..789850	-	369	WP_235399448.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L1P14_RS03855 (790013)	790013..790234	-	222	WP_000692345.1	DUF987 domain-containing protein	-
L1P14_RS03860 (790297)	790297..790773	-	477	WP_001186727.1	RadC family protein	-
L1P14_RS03865 (790789)	790789..791274	-	486	WP_160392016.1	antirestriction protein	-
L1P14_RS03870 (791329)	791329..792147	-	819	WP_235418533.1	DUF932 domain-containing protein	-
L1P14_RS03875 (792247)	792247..792480	-	234	WP_001119729.1	DUF905 family protein	-
L1P14_RS03880 (792559)	792559..793014	-	456	WP_000581504.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13991.96 Da
• Isoelectric Point: 7.8045

>T232481 WP_032286705.1 NZ_CP091029:c789392-789015 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T232481 NZ_CP127306:1750074-1750601 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13582.36 Da
• Isoelectric Point: 6.6255

>AT232481 WP_235399448.1 NZ_CP091029:c789850-789482 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYSTPETKK

Nucleotide

• Length: 369 bp

>AT232481 NZ_CP127306:1749801-1750067 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA