Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 291513..292313 Replicon chromosome
Accession NZ_CP091029
Organism Escherichia coli strain STEC384

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag L1P14_RS01355 Protein ID WP_000342449.1
Coordinates 291786..292313 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag L1P14_RS01350 Protein ID WP_001277108.1
Coordinates 291513..291779 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P14_RS01330 (287171) 287171..287839 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
L1P14_RS01335 (287832) 287832..288890 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
L1P14_RS01340 (289135) 289135..289989 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
L1P14_RS01345 (290260) 290260..291363 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
L1P14_RS01350 (291513) 291513..291779 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
L1P14_RS01355 (291786) 291786..292313 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
L1P14_RS01360 (292310) 292310..292693 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
L1P14_RS01365 (293117) 293117..294226 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
L1P14_RS01370 (294274) 294274..295200 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
L1P14_RS01375 (295197) 295197..296474 + 1278 WP_000803784.1 branched chain amino acid ABC transporter permease LivM -
L1P14_RS01380 (296471) 296471..297238 + 768 WP_000082103.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T232479 WP_000342449.1 NZ_CP091029:291786-292313 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T232479 NZ_CP127306:c1243212-1242901 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATTTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT232479 WP_001277108.1 NZ_CP091029:291513-291779 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT232479 NZ_CP127306:c1242900-1242610 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References