Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 270223..270809 Replicon chromosome
Accession NZ_CP091029
Organism Escherichia coli strain STEC384

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag L1P14_RS01230 Protein ID WP_042014162.1
Coordinates 270223..270453 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag L1P14_RS01235 Protein ID WP_000593555.1
Coordinates 270450..270809 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P14_RS01220 (266260) 266260..268995 + 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -
L1P14_RS01225 (268995) 268995..270119 + 1125 WP_001314210.1 ABC transporter permease -
L1P14_RS01230 (270223) 270223..270453 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
L1P14_RS01235 (270450) 270450..270809 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
L1P14_RS01240 (270929) 270929..271330 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
L1P14_RS01245 (271336) 271336..272142 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
L1P14_RS01250 (272139) 272139..272903 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
L1P14_RS01255 (272903) 272903..273736 - 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
L1P14_RS01260 (273733) 273733..274677 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T232478 WP_042014162.1 NZ_CP091029:270223-270453 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T232478 NZ_CP127306:c966120-965947 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT232478 WP_000593555.1 NZ_CP091029:270450-270809 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT232478 NZ_CP127306:c966444-966148 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References