Detailed information of TA system TA232471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 84617..85164
• Replicon: plasmid p1_STEC383
• Accession: NZ_CP091027
• Organism: Escherichia coli strain STEC383

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: L1P12_RS25660
• Protein ID: WP_248699117.1
• Coordinates: 84617..84895 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: L1P12_RS25665
• Protein ID: WP_000079941.1
• Coordinates: 84895..85164 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P12_RS25630 (81308)	81308..81469	+	162	Protein_100	IS66 family transposase	-
L1P12_RS25635 (81467)	81467..82039	-	573	Protein_101	IS21 family transposase	-
L1P12_RS25640 (82096)	82096..83118	+	1023	WP_063081680.1	IS21-like element IS100 family transposase	-
L1P12_RS25645 (83115)	83115..83897	+	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-
L1P12_RS25650 (83963)	83963..84364	-	402	Protein_104	IS21 family transposase	-
L1P12_RS25655 (84452)	84452..84571	-	120	Protein_105	3'-5' exonuclease	-
L1P12_RS25660 (84617)	84617..84895	-	279	WP_248699117.1	type II toxin-antitoxin system toxin YacB	Toxin
L1P12_RS25665 (84895)	84895..85164	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
L1P12_RS25670 (86077)	86077..86934	-	858	WP_136769174.1	incFII family plasmid replication initiator RepA	-
L1P12_RS25675 (86927)	86927..87409	-	483	WP_001523473.1	hypothetical protein	-
L1P12_RS25680 (87402)	87402..87449	-	48	WP_229471593.1	hypothetical protein	-
L1P12_RS25685 (87440)	87440..87691	+	252	WP_223195197.1	replication protein RepA	-
L1P12_RS25690 (87708)	87708..87965	-	258	WP_000083845.1	replication regulatory protein RepA	-
L1P12_RS25695 (88249)	88249..88398	-	150	WP_001312851.1	Hok/Gef family protein	-
- (88442)	88442..88503	+	62	NuclAT_1	-	-
- (88442)	88442..88503	+	62	NuclAT_1	-	-
- (88442)	88442..88503	+	62	NuclAT_1	-	-
- (88442)	88442..88503	+	62	NuclAT_1	-	-
L1P12_RS25700 (88759)	88759..88833	-	75	Protein_114	endonuclease	-
L1P12_RS25705 (89024)	89024..89221	-	198	WP_097464675.1	hypothetical protein	-
L1P12_RS25710 (89357)	89357..89917	-	561	WP_001587438.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / ant(3'')-Ia / dfrA1 / tet(A) / sul3	-	1..111805	111805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10789.40 Da
• Isoelectric Point: 8.4615

>T232471 WP_248699117.1 NZ_CP091027:c84895-84617 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDNQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T232471 NZ_CP127303:4015577-4015684 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT232471 WP_000079941.1 NZ_CP091027:c85164-84895 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT232471 NZ_CP127303:c4015530-4015464 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5