Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 55052..55610 Replicon plasmid p1_STEC383
Accession NZ_CP091027
Organism Escherichia coli strain STEC383

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag L1P12_RS25485 Protein ID WP_235387476.1
Coordinates 55052..55390 (-) Length 113 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag L1P12_RS25490 Protein ID WP_000813634.1
Coordinates 55392..55610 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P12_RS25460 (51125) 51125..51769 - 645 WP_001477618.1 replication initiation protein RepE -
L1P12_RS25465 (51816) 51816..52622 - 807 WP_235387475.1 site-specific integrase -
L1P12_RS25470 (52688) 52688..54226 - 1539 WP_000099160.1 IS66-like element ISEc22 family transposase -
L1P12_RS25475 (54275) 54275..54622 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
L1P12_RS25480 (54619) 54619..55023 - 405 WP_096169218.1 transposase -
L1P12_RS25485 (55052) 55052..55390 - 339 WP_235387476.1 type II toxin-antitoxin system toxin CcdB Toxin
L1P12_RS25490 (55392) 55392..55610 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L1P12_RS25495 (56167) 56167..59982 - 3816 WP_235387477.1 hypothetical protein -
L1P12_RS25500 (60208) 60208..60393 + 186 Protein_74 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / ant(3'')-Ia / dfrA1 / tet(A) / sul3 - 1..111805 111805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-95)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 13170.25 Da        Isoelectric Point: 10.0891

>T232470 WP_235387476.1 NZ_CP091027:c55390-55052 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINRKRRLSTVWQILKFLRE

Download         Length: 339 bp

>T232470 NZ_CP127303:3406816-3406919 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT232470 WP_000813634.1 NZ_CP091027:c55610-55392 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT232470 NZ_CP127303:c3406957-3406686 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References