Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2566046..2566684 Replicon chromosome
Accession NZ_CP091026
Organism Escherichia coli strain STEC383

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag L1P12_RS12400 Protein ID WP_000813794.1
Coordinates 2566508..2566684 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag L1P12_RS12395 Protein ID WP_001270286.1
Coordinates 2566046..2566462 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P12_RS12375 (2561198) 2561198..2562139 - 942 WP_032177241.1 ABC transporter permease -
L1P12_RS12380 (2562140) 2562140..2563153 - 1014 WP_032177240.1 ABC transporter ATP-binding protein -
L1P12_RS12385 (2563171) 2563171..2564316 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
L1P12_RS12390 (2564561) 2564561..2565967 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
L1P12_RS12395 (2566046) 2566046..2566462 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
L1P12_RS12400 (2566508) 2566508..2566684 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
L1P12_RS12405 (2566906) 2566906..2567136 + 231 WP_000494244.1 YncJ family protein -
L1P12_RS12410 (2567228) 2567228..2569189 - 1962 WP_001515270.1 23S rRNA 5-hydroxycytidine C2501 synthase -
L1P12_RS12415 (2569262) 2569262..2569798 - 537 WP_000429150.1 DNA-binding transcriptional regulator SutR -
L1P12_RS12420 (2569890) 2569890..2571065 + 1176 WP_235387310.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2571105..2572253 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T232462 WP_000813794.1 NZ_CP091026:c2566684-2566508 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T232462 NZ_CP127303:c1013480-1013373 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT232462 WP_001270286.1 NZ_CP091026:c2566462-2566046 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT232462 NZ_CP127303:1013528-1013594 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References