Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2486152..2487740 Replicon chromosome
Accession NZ_CP091026
Organism Escherichia coli strain STEC383

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag L1P12_RS12070 Protein ID WP_001125439.1
Coordinates 2486418..2487740 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag L1P12_RS12065 Protein ID WP_001301023.1
Coordinates 2486152..2486418 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P12_RS12050 (2482927) 2482927..2483907 + 981 WP_000019440.1 IS5-like element ISKpn26 family transposase -
L1P12_RS12055 (2484150) 2484150..2485679 + 1530 WP_235387462.1 hypothetical protein -
L1P12_RS12060 (2485645) 2485645..2485929 + 285 WP_141035754.1 autotransporter outer membrane beta-barrel domain-containing protein -
L1P12_RS12065 (2486152) 2486152..2486418 + 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
L1P12_RS12070 (2486418) 2486418..2487740 + 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
L1P12_RS12075 (2488023) 2488023..2488790 + 768 Protein_2368 fimbrial protein -
L1P12_RS12080 (2489124) 2489124..2491403 + 2280 WP_000726695.1 acid resistance putative oxidoreductase YdeP -
L1P12_RS12085 (2491651) 2491651..2491848 + 198 WP_000543394.1 two-component system connector SafA -
L1P12_RS12090 (2491922) 2491922..2492683 + 762 WP_000060500.1 acid stress response transcriptional regulator YdeO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2482927..2499151 16224
- flank IS/Tn - - 2482927..2483907 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T232461 WP_001125439.1 NZ_CP091026:2486418-2487740 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T232461 NZ_CP127303:c1012998-1012891 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT232461 WP_001301023.1 NZ_CP091026:2486152-2486418 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT232461 NZ_CP127303:1013046-1013112 [Escherichia coli]
GGTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References