Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1957184..1957952 Replicon chromosome
Accession NZ_CP091026
Organism Escherichia coli strain STEC383

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag L1P12_RS09380 Protein ID WP_000854814.1
Coordinates 1957184..1957558 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3W4SUB1
Locus tag L1P12_RS09385 Protein ID WP_044863316.1
Coordinates 1957647..1957952 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P12_RS09340 (1952580) 1952580..1953746 + 1167 WP_001464461.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
L1P12_RS09345 (1953865) 1953865..1954338 + 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
L1P12_RS09350 (1954536) 1954536..1955594 + 1059 WP_001200891.1 FUSC family protein -
L1P12_RS09355 (1955766) 1955766..1956095 + 330 WP_000450409.1 DUF496 family protein -
L1P12_RS09360 (1956196) 1956196..1956330 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
L1P12_RS09365 (1956450) 1956450..1956578 + 129 Protein_1836 transposase domain-containing protein -
L1P12_RS09370 (1956867) 1956867..1956947 - 81 Protein_1837 hypothetical protein -
L1P12_RS09375 (1956993) 1956993..1957187 - 195 WP_000988600.1 DUF5983 family protein -
L1P12_RS09380 (1957184) 1957184..1957558 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
L1P12_RS09385 (1957647) 1957647..1957952 - 306 WP_044863316.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
L1P12_RS09390 (1958089) 1958089..1958310 - 222 WP_000692323.1 DUF987 domain-containing protein -
L1P12_RS09395 (1958373) 1958373..1958849 - 477 WP_001186726.1 RadC family protein -
L1P12_RS09400 (1958865) 1958865..1959344 - 480 WP_000860087.1 antirestriction protein -
L1P12_RS09405 (1959426) 1959426..1960244 - 819 WP_001234629.1 DUF932 domain-containing protein -
L1P12_RS09410 (1960344) 1960344..1960577 - 234 WP_001119729.1 DUF905 family protein -
L1P12_RS09415 (1960656) 1960656..1961111 - 456 WP_000581504.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(2-96)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T232452 WP_000854814.1 NZ_CP091026:c1957558-1957184 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T232452 NZ_CP127301:162430-162579 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11250.00 Da        Isoelectric Point: 7.2621

>AT232452 WP_044863316.1 NZ_CP091026:c1957952-1957647 [Escherichia coli]
MPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKA
DTLSSCGYVYLAVYPTPEMKN

Download         Length: 306 bp

>AT232452 NZ_CP127301:c162386-162325 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A3W4SUB1

References