Detailed information of TA system TA023245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 53264..53814
• Replicon: chromosome
• Accession: NC_011601
• Organism: Escherichia coli O127:H6 str. E2348/69

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PDG4
• Locus tag: E2348C_RS00265
• Protein ID: WP_001160966.1
• Coordinates: 53500..53814 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: E2348C_RS00260
• Protein ID: WP_000125566.1
• Coordinates: 53264..53497 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2348C_RS00235 (E2348_C_0045)	48337..48624	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
E2348C_RS00240 (E2348_C_0046)	48683..50014	+	1332	WP_001183210.1	MFS transporter	-
E2348C_RS00245 (E2348_C_0047)	50122..50652	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
E2348C_RS00250 (E2348_C_0048)	50645..52507	+	1863	WP_000377124.1	glutathione-regulated potassium-efflux system protein KefC	-
E2348C_RS00255 (E2348_C_0049)	52699..53178	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
E2348C_RS00260 (E2348_C_0050)	53264..53497	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
E2348C_RS00265 (E2348_C_0051)	53500..53814	+	315	WP_001160966.1	CcdB family protein	Toxin
E2348C_RS00270 (E2348_C_0052)	53811..54659	-	849	WP_000257182.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
E2348C_RS00275 (E2348_C_0053)	54666..55043	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
E2348C_RS00280 (E2348_C_0054)	55046..55867	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
E2348C_RS00285 (E2348_C_0055)	55864..56853	-	990	WP_000241226.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
E2348C_RS00290 (E2348_C_0056)	56853..58139	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.72 Da
• Isoelectric Point: 9.0851

>T23245 WP_001160966.1 NC_011601:53500-53814 [Escherichia coli O127:H6 str. E2348/69]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T23245 NC_011601:53500-53814 [Escherichia coli O127:H6 str. E2348/69]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT23245 WP_000125566.1 NC_011601:53264-53497 [Escherichia coli O127:H6 str. E2348/69]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT23245 NC_011601:53264-53497 [Escherichia coli O127:H6 str. E2348/69]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A829G815

Antitoxin

• AlphaFold DB: A0A7U9LTM9