Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 980477..981131 Replicon chromosome
Accession NZ_CP091026
Organism Escherichia coli strain STEC383

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L1P12_RS04740 Protein ID WP_000244777.1
Coordinates 980724..981131 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L1P12_RS04735 Protein ID WP_000354046.1
Coordinates 980477..980743 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P12_RS04710 (975647) 975647..976389 + 743 Protein_926 SDR family oxidoreductase -
L1P12_RS04715 (976446) 976446..977879 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
L1P12_RS04720 (977924) 977924..978235 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
L1P12_RS04725 (978399) 978399..979058 + 660 WP_000250274.1 hemolysin III family protein -
L1P12_RS04730 (979254) 979254..980234 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L1P12_RS04735 (980477) 980477..980743 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L1P12_RS04740 (980724) 980724..981131 + 408 WP_000244777.1 protein YgfX Toxin
L1P12_RS04745 (981171) 981171..981692 - 522 WP_001055874.1 flavodoxin FldB -
L1P12_RS04750 (981804) 981804..982700 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L1P12_RS04755 (982725) 982725..983435 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L1P12_RS04760 (983441) 983441..985174 + 1734 WP_096111170.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T232448 WP_000244777.1 NZ_CP091026:980724-981131 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T232448 NZ_CP127301:c94964-94548 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT232448 WP_000354046.1 NZ_CP091026:980477-980743 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT232448 NZ_CP127301:c95191-94961 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGAGCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References