Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 853820..854652 Replicon chromosome
Accession NZ_CP091026
Organism Escherichia coli strain STEC383

Toxin (Protein)


Gene name yeeV Uniprot ID A0A346GNK2
Locus tag L1P12_RS04115 Protein ID WP_032082789.1
Coordinates 853820..854194 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M2U6P5
Locus tag L1P12_RS04120 Protein ID WP_001774606.1
Coordinates 854284..854652 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P12_RS04085 (848923) 848923..849699 + 777 WP_235387434.1 type II secretion system protein GspL -
L1P12_RS04090 (849701) 849701..850237 + 537 WP_059328842.1 GspM family type II secretion system protein YghD -
L1P12_RS04095 (850573) 850573..852108 - 1536 WP_001609560.1 EAL domain-containing protein -
L1P12_RS04100 (852179) 852179..853024 - 846 WP_032082786.1 DUF4942 domain-containing protein -
L1P12_RS04105 (853121) 853121..853318 - 198 WP_032082787.1 DUF957 domain-containing protein -
L1P12_RS04110 (853335) 853335..853823 - 489 WP_032082788.1 DUF5983 family protein -
L1P12_RS04115 (853820) 853820..854194 - 375 WP_032082789.1 TA system toxin CbtA family protein Toxin
L1P12_RS04120 (854284) 854284..854652 - 369 WP_001774606.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
L1P12_RS04125 (854702) 854702..855346 - 645 WP_001774605.1 hypothetical protein -
L1P12_RS04130 (855365) 855365..855586 - 222 WP_000692312.1 DUF987 domain-containing protein -
L1P12_RS04135 (855649) 855649..856125 - 477 WP_001297237.1 RadC family protein -
L1P12_RS04140 (856141) 856141..856620 - 480 WP_032082790.1 antirestriction protein -
L1P12_RS04145 (856714) 856714..856959 - 246 WP_032082791.1 hypothetical protein -
L1P12_RS04150 (856959) 856959..857777 - 819 WP_044154198.1 DUF932 domain-containing protein -
L1P12_RS04155 (857876) 857876..858109 - 234 WP_001278287.1 DUF905 family protein -
L1P12_RS04160 (858115) 858115..858792 - 678 WP_001097305.1 hypothetical protein -
L1P12_RS04165 (858940) 858940..859620 - 681 WP_032082725.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 850391..911118 60727


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14214.29 Da        Isoelectric Point: 8.2905

>T232447 WP_032082789.1 NZ_CP091026:c854194-853820 [Escherichia coli]
MKTLPDTHVREASRCPPPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRTTGLMTRDNYRTVNNITLSKHPEKR

Download         Length: 375 bp

>T232447 NZ_CP127300:c53138-52836 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAATGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.31 Da        Isoelectric Point: 5.8746

>AT232447 WP_001774606.1 NZ_CP091026:c854652-854284 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT232447 NZ_CP127300:c52765-52427 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A346GNK2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2U6P5

References