Detailed information of TA system TA232441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 55506..56065
• Replicon: plasmid p_STEC379
• Accession: NZ_CP091025
• Organism: Escherichia coli strain STEC379

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L1P13_RS25065
• Protein ID: WP_032084131.1
• Coordinates: 55790..56065 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L1P13_RS25060
• Protein ID: WP_032084130.1
• Coordinates: 55506..55790 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P13_RS25035 (52910)	52910..53602	+	693	WP_062886836.1	fimbrial chaperone	-
L1P13_RS25040 (53718)	53718..54095	+	378	WP_235399558.1	hypothetical protein	-
L1P13_RS25045 (54246)	54246..55088	+	843	WP_064578920.1	hypothetical protein	-
L1P13_RS25050 (55144)	55144..55269	-	126	Protein_68	IS256 family transposase	-
L1P13_RS25055 (55341)	55341..55421	-	81	Protein_69	ATP-binding protein	-
L1P13_RS25060 (55506)	55506..55790	+	285	WP_032084130.1	ribbon-helix-helix domain-containing protein	Antitoxin
L1P13_RS25065 (55790)	55790..56065	+	276	WP_032084131.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L1P13_RS25070 (56171)	56171..56374	+	204	Protein_72	hypothetical protein	-
L1P13_RS25075 (56722)	56722..57069	+	348	WP_235399559.1	DUF6404 family protein	-
L1P13_RS25080 (57087)	57087..57521	-	435	Protein_74	hypothetical protein	-
L1P13_RS25085 (57805)	57805..58728	-	924	WP_032084040.1	hypothetical protein	-
L1P13_RS25090 (59621)	59621..60394	-	774	WP_032084041.1	YaiO family outer membrane beta-barrel protein	-
L1P13_RS25095 (60396)	60396..60866	-	471	WP_235399562.1	acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa	1..71807	71807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10765.45 Da
• Isoelectric Point: 9.9324

>T232441 WP_032084131.1 NZ_CP091025:55790-56065 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFSGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T232441 NZ_CP127297:4025817-4026359 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10517.92 Da
• Isoelectric Point: 5.6558

>AT232441 WP_032084130.1 NZ_CP091025:55506-55790 [Escherichia coli]
MQMKNNSAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQTW
ADSLNTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT232441 NZ_CP127297:4025522-4025833 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA