Detailed information of TA system TA232434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3780969..3781806
• Replicon: chromosome
• Accession: NZ_CP091024
• Organism: Escherichia coli strain STEC379

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: L1P13_RS18810
• Protein ID: WP_000227784.1
• Coordinates: 3781264..3781806 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: L1P13_RS18805
• Protein ID: WP_001297137.1
• Coordinates: 3780969..3781280 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P13_RS18780 (3775989)	3775989..3776936	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
L1P13_RS18785 (3776958)	3776958..3778949	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
L1P13_RS18790 (3778939)	3778939..3779553	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
L1P13_RS18795 (3779553)	3779553..3779882	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
L1P13_RS18800 (3779894)	3779894..3780784	+	891	WP_000971336.1	heme o synthase	-
L1P13_RS18805 (3780969)	3780969..3781280	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
L1P13_RS18810 (3781264)	3781264..3781806	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
L1P13_RS18815 (3781862)	3781862..3782797	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
L1P13_RS18820 (3783205)	3783205..3784569	+	1365	WP_001000975.1	MFS transporter	-
L1P13_RS18825 (3784697)	3784697..3785188	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
L1P13_RS18830 (3785356)	3785356..3786267	+	912	WP_000705877.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T232434 WP_000227784.1 NZ_CP091024:3781264-3781806 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T232434 NZ_CP127297:2761585-2761872 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAAGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT232434 WP_001297137.1 NZ_CP091024:3780969-3781280 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT232434 NZ_CP127297:2761346-2761585 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3