Detailed information of TA system TA232409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 3679..4226
• Replicon: plasmid p_STEC276
• Accession: NZ_CP091023
• Organism: Escherichia coli strain STEC276

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: L1O93_RS23635
• Protein ID: WP_001384452.1
• Coordinates: 3679..3957 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: L1O93_RS23640
• Protein ID: WP_032307223.1
• Coordinates: 3957..4226 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O93_RS23615 (1)	1..1417	+	1417	Protein_0	Tn3-like element TnAs1 family transposase	-
L1O93_RS23620 (1458)	1458..1724	+	267	WP_000627831.1	hypothetical protein	-
L1O93_RS23625 (2201)	2201..2650	-	450	WP_001236003.1	hypothetical protein	-
L1O93_RS23630 (2791)	2791..3639	-	849	WP_001057996.1	3'-5' exonuclease	-
L1O93_RS23635 (3679)	3679..3957	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
L1O93_RS23640 (3957)	3957..4226	-	270	WP_032307223.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
L1O93_RS23645 (4281)	4281..4439	-	159	WP_000841008.1	hypothetical protein	-
L1O93_RS23650 (5139)	5139..5996	-	858	WP_001401515.1	incFII family plasmid replication initiator RepA	-
L1O93_RS24340 (5989)	5989..6063	-	75	WP_001375168.1	RepA leader peptide Tap	-
L1O93_RS23665 (6297)	6297..6554	-	258	WP_032082917.1	replication regulatory protein RepA	-
L1O93_RS23670 (6794)	6794..7384	-	591	WP_032082916.1	DUF2726 domain-containing protein	-
L1O93_RS23675 (7422)	7422..7631	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
L1O93_RS23680 (7677)	7677..8138	-	462	WP_021545193.1	thermonuclease family protein	-
L1O93_RS23685 (8437)	8437..8595	-	159	WP_021545194.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A)	estIa	1..87231	87231
	-	flank	IS/Tn	-	-	8..1417	1409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T232409 WP_001384452.1 NZ_CP091023:c3957-3679 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T232409 NZ_CP127277:2912402-2912842 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10177.47 Da
• Isoelectric Point: 6.7251

>AT232409 WP_032307223.1 NZ_CP091023:c4226-3957 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGHQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT232409 NZ_CP127277:2912128-2912415 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829CL36