Detailed information of TA system TA232393

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1873892..1874727
• Replicon: chromosome
• Accession: NZ_CP091022
• Organism: Escherichia coli strain STEC276

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: L1O93_RS08845
• Protein ID: WP_020239660.1
• Coordinates: 1873892..1874269 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: L1O93_RS08850
• Protein ID: WP_001295723.1
• Coordinates: 1874359..1874727 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O93_RS08810 (1869412)	1869412..1869885	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
L1O93_RS08815 (1870083)	1870083..1871141	+	1059	WP_001200895.1	FUSC family protein	-
L1O93_RS08820 (1871313)	1871313..1871642	+	330	WP_000450409.1	DUF496 family protein	-
L1O93_RS08825 (1871743)	1871743..1872108	-	366	WP_001280454.1	EutP/PduV family microcompartment system protein	-
L1O93_RS08830 (1872379)	1872379..1872768	-	390	WP_077875581.1	transposase	-
L1O93_RS08835 (1873566)	1873566..1873646	-	81	Protein_1737	hypothetical protein	-
L1O93_RS08840 (1873746)	1873746..1873895	-	150	Protein_1738	DUF5983 family protein	-
L1O93_RS08845 (1873892)	1873892..1874269	-	378	WP_020239660.1	TA system toxin CbtA family protein	Toxin
L1O93_RS08850 (1874359)	1874359..1874727	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L1O93_RS08855 (1874890)	1874890..1875111	-	222	WP_000692345.1	DUF987 domain-containing protein	-
L1O93_RS08860 (1875174)	1875174..1875650	-	477	WP_001186775.1	RadC family protein	-
L1O93_RS08865 (1875666)	1875666..1876139	-	474	WP_001765426.1	antirestriction protein	-
L1O93_RS08870 (1876481)	1876481..1877299	-	819	WP_020239663.1	DUF932 domain-containing protein	-
L1O93_RS08875 (1877417)	1877417..1877612	-	196	Protein_1745	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14135.16 Da
• Isoelectric Point: 7.3249

>T232393 WP_020239660.1 NZ_CP091022:c1874269-1873892 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLARLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T232393 NZ_CP127277:1958334-1958582 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT232393 WP_001295723.1 NZ_CP091022:c1874727-1874359 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT232393 NZ_CP127277:1958122-1958334 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: S1NM52