Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 934076..934730 Replicon chromosome
Accession NZ_CP091022
Organism Escherichia coli strain STEC276

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L1O93_RS04490 Protein ID WP_000244777.1
Coordinates 934323..934730 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L1O93_RS04485 Protein ID WP_000354046.1
Coordinates 934076..934342 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O93_RS04465 (930045) 930045..931478 - 1434 WP_240992398.1 6-phospho-beta-glucosidase BglA -
L1O93_RS04470 (931523) 931523..931834 + 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
L1O93_RS04475 (931998) 931998..932657 + 660 WP_000250274.1 hemolysin III family protein -
L1O93_RS04480 (932853) 932853..933833 - 981 WP_001514522.1 tRNA-modifying protein YgfZ -
L1O93_RS04485 (934076) 934076..934342 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L1O93_RS04490 (934323) 934323..934730 + 408 WP_000244777.1 protein YgfX Toxin
L1O93_RS04495 (934770) 934770..935291 - 522 WP_001055874.1 flavodoxin FldB -
L1O93_RS04500 (935403) 935403..936299 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L1O93_RS04505 (936324) 936324..937034 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L1O93_RS04510 (937040) 937040..938773 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T232390 WP_000244777.1 NZ_CP091022:934323-934730 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T232390 NZ_CP127277:1686205-1686522 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT232390 WP_000354046.1 NZ_CP091022:934076-934342 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT232390 NZ_CP127277:1685906-1686208 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References