Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4711603..4712100 Replicon chromosome
Accession NZ_CP091020
Organism Escherichia coli strain STEC308

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L1O95_RS23780 Protein ID WP_001173343.1
Coordinates 4711603..4711776 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag L1O95_RS23785 Protein ID WP_001425728.1
Coordinates 4711804..4712100 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O95_RS23760 (4706937) 4706937..4708394 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
L1O95_RS23765 (4708631) 4708631..4709416 + 786 Protein_4664 OB-fold nucleic acid binding domain-containing protein -
L1O95_RS23775 (4710747) 4710747..4711484 + 738 Protein_4666 lysine--tRNA ligase -
L1O95_RS23780 (4711603) 4711603..4711776 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L1O95_RS23785 (4711804) 4711804..4712100 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L1O95_RS23790 (4712327) 4712327..4712599 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
L1O95_RS23795 (4712611) 4712611..4712841 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
L1O95_RS23800 (4713022) 4713022..4714653 + 1632 WP_001216487.1 sensor histidine kinase -
L1O95_RS23805 (4714650) 4714650..4715369 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4699463..4712100 12637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T232384 WP_001173343.1 NZ_CP091020:c4711776-4711603 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T232384 NZ_CP127277:c1230608-1230297 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT232384 WP_001425728.1 NZ_CP091020:c4712100-4711804 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT232384 NZ_CP127277:c1230928-1230608 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References