Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4544966..4545561 Replicon chromosome
Accession NZ_CP091020
Organism Escherichia coli strain STEC308

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag L1O95_RS22955 Protein ID WP_000239579.1
Coordinates 4544966..4545316 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag L1O95_RS22960 Protein ID WP_001223208.1
Coordinates 4545310..4545561 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O95_RS22935 (4540242) 4540242..4541264 - 1023 WP_001339490.1 ABC transporter permease -
L1O95_RS22940 (4541278) 4541278..4542780 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
L1O95_RS22945 (4543090) 4543090..4544046 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
L1O95_RS22950 (4544356) 4544356..4544886 + 531 WP_000055072.1 inorganic diphosphatase -
L1O95_RS22955 (4544966) 4544966..4545316 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
L1O95_RS22960 (4545310) 4545310..4545561 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
L1O95_RS22965 (4545774) 4545774..4546115 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
L1O95_RS22970 (4546118) 4546118..4549897 - 3780 WP_000060921.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T232382 WP_000239579.1 NZ_CP091020:c4545316-4544966 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T232382 NZ_CP127277:1014503-1014937 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT232382 WP_001223208.1 NZ_CP091020:c4545561-4545310 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT232382 NZ_CP127277:1014318-1014497 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References