Detailed information of TA system TA232366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1481203..1481828
• Replicon: chromosome
• Accession: NZ_CP091020
• Organism: Escherichia coli strain STEC308

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L1O95_RS07300
• Protein ID: WP_000911330.1
• Coordinates: 1481430..1481828 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: L1O95_RS07295
• Protein ID: WP_000450524.1
• Coordinates: 1481203..1481430 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O95_RS07270 (1477006)	1477006..1477476	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
L1O95_RS07275 (1477476)	1477476..1478048	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
L1O95_RS07280 (1478194)	1478194..1479072	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
L1O95_RS07285 (1479089)	1479089..1480123	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
L1O95_RS07290 (1480336)	1480336..1481049	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
L1O95_RS07295 (1481203)	1481203..1481430	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
L1O95_RS07300 (1481430)	1481430..1481828	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
L1O95_RS07305 (1481975)	1481975..1482838	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
L1O95_RS07310 (1482853)	1482853..1484868	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
L1O95_RS07315 (1484942)	1484942..1485640	+	699	WP_000679823.1	esterase	-
L1O95_RS07320 (1485750)	1485750..1485950	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T232366 WP_000911330.1 NZ_CP091020:1481430-1481828 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T232366 NZ_CP127277:363113-363415 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT232366 WP_000450524.1 NZ_CP091020:1481203-1481430 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT232366 NZ_CP127277:363463-363684 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33