Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 995106..995760 Replicon chromosome
Accession NZ_CP091020
Organism Escherichia coli strain STEC308

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L1O95_RS04895 Protein ID WP_000244777.1
Coordinates 995353..995760 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L1O95_RS04890 Protein ID WP_000354046.1
Coordinates 995106..995372 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O95_RS04865 (990394) 990394..991137 + 744 WP_000951948.1 SDR family oxidoreductase -
L1O95_RS04870 (991194) 991194..992627 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
L1O95_RS04875 (992672) 992672..992983 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
L1O95_RS04880 (993147) 993147..993806 + 660 WP_000250274.1 hemolysin III family protein -
L1O95_RS04885 (993883) 993883..994863 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L1O95_RS04890 (995106) 995106..995372 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L1O95_RS04895 (995353) 995353..995760 + 408 WP_000244777.1 protein YgfX Toxin
L1O95_RS04900 (995800) 995800..996321 - 522 WP_001055874.1 flavodoxin FldB -
L1O95_RS04905 (996433) 996433..997329 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L1O95_RS04910 (997354) 997354..998064 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L1O95_RS04915 (998070) 998070..999803 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T232363 WP_000244777.1 NZ_CP091020:995353-995760 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T232363 NZ_CP127276:3811257-3811667 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT232363 WP_000354046.1 NZ_CP091020:995106-995372 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT232363 NZ_CP127276:3810982-3811260 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCTGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References