Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 272189..272775 Replicon chromosome
Accession NZ_CP091020
Organism Escherichia coli strain STEC308

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag L1O95_RS01240 Protein ID WP_042014162.1
Coordinates 272189..272419 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag L1O95_RS01245 Protein ID WP_000593555.1
Coordinates 272416..272775 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O95_RS01230 (268226) 268226..270961 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
L1O95_RS01235 (270961) 270961..272085 + 1125 WP_001314210.1 ABC transporter permease -
L1O95_RS01240 (272189) 272189..272419 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
L1O95_RS01245 (272416) 272416..272775 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
L1O95_RS01250 (272895) 272895..273296 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
L1O95_RS01255 (273302) 273302..274108 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
L1O95_RS01260 (274105) 274105..274869 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
L1O95_RS01265 (274869) 274869..275702 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
L1O95_RS01270 (275699) 275699..276643 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T232358 WP_042014162.1 NZ_CP091020:272189-272419 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T232358 NZ_CP127276:3168091-3168534 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT232358 WP_000593555.1 NZ_CP091020:272416-272775 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT232358 NZ_CP127276:3167847-3168104 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References