Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4711607..4712104 Replicon chromosome
Accession NZ_CP091018
Organism Escherichia coli strain STEC307

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L1O94_RS23770 Protein ID WP_001173343.1
Coordinates 4711607..4711780 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag L1O94_RS23775 Protein ID WP_001425728.1
Coordinates 4711808..4712104 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O94_RS23750 (4706941) 4706941..4708398 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
L1O94_RS23755 (4708635) 4708635..4709420 + 786 Protein_4663 OB-fold nucleic acid binding domain-containing protein -
L1O94_RS23765 (4710751) 4710751..4711488 + 738 Protein_4665 lysine--tRNA ligase -
L1O94_RS23770 (4711607) 4711607..4711780 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L1O94_RS23775 (4711808) 4711808..4712104 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L1O94_RS23780 (4712331) 4712331..4712603 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
L1O94_RS23785 (4712615) 4712615..4712845 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
L1O94_RS23790 (4713026) 4713026..4714657 + 1632 WP_001216487.1 sensor histidine kinase -
L1O94_RS23795 (4714654) 4714654..4715373 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4699467..4712104 12637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T232356 WP_001173343.1 NZ_CP091018:c4711780-4711607 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T232356 NZ_CP127276:c3121721-3121329 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT232356 WP_001425728.1 NZ_CP091018:c4712104-4711808 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT232356 NZ_CP127276:c3121933-3121718 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References