Detailed information of TA system TA023235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 176869..177563
• Replicon: chromosome
• Accession: NC_011567
• Organism: Anoxybacillus flavithermus WK1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B7GJ05
• Locus tag: AFLV_RS01005
• Protein ID: WP_012573896.1
• Coordinates: 177177..177563 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B7GJ04
• Locus tag: AFLV_RS01000
• Protein ID: WP_012573895.1
• Coordinates: 176869..177177 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFLV_RS00985	173542..175209	+	1668	WP_148205011.1	putative DNA binding domain-containing protein	-
AFLV_RS00990	175588..175824	+	237	WP_041637620.1	hypothetical protein	-
AFLV_RS14960	176122..176286	-	165	WP_080513047.1	transposase	-
AFLV_RS00995	176376..176591	+	216	Protein_167	helix-turn-helix domain-containing protein	-
AFLV_RS01000	176869..177177	+	309	WP_012573895.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AFLV_RS01005	177177..177563	+	387	WP_012573896.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AFLV_RS01010	178378..178728	+	351	WP_041637622.1	hypothetical protein	-
AFLV_RS01015	178743..179366	+	624	WP_041637624.1	PspA/IM30 family protein	-
AFLV_RS01025	179605..179882	+	278	Protein_172	transposase	-
AFLV_RS01030	180021..180746	+	726	WP_041637632.1	cell wall-active antibiotics response protein	-
AFLV_RS01035	180743..181750	+	1008	WP_012573900.1	sensor histidine kinase	-
AFLV_RS01040	181763..182389	+	627	WP_012573901.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-116)

Antitoxin

• SpoVT-AbrB domain (26-71)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14299.47 Da
• Isoelectric Point: 4.9366

>T23235 WP_012573896.1 NC_011567:177177-177563 [Anoxybacillus flavithermus WK1]
MPVDVPGRGDFIVINFDPQAGHEQAGRRNAIVLSPKEFNQATGFIAVCPITNQKKGYPFEVELPKKGIVLDGDGYPITGV
ILTDQIKSLDWKARNLKILKKYNPEDAQIEEIDKIIDECLAKIETYLT

Nucleotide

• Length: 387 bp

>T23235 NC_011567:177177-177563 [Anoxybacillus flavithermus WK1]
ATGCCAGTAGATGTGCCAGGGAGAGGCGATTTTATCGTTATCAACTTCGATCCACAAGCTGGTCATGAACAGGCTGGGAG
AAGGAATGCTATTGTTTTGTCGCCTAAAGAATTCAATCAAGCAACAGGATTTATAGCTGTTTGTCCAATTACTAATCAAA
AGAAAGGTTATCCTTTTGAAGTGGAATTACCAAAAAAAGGTATAGTTCTTGATGGCGATGGTTATCCAATAACGGGGGTA
ATTTTAACCGATCAAATTAAATCATTGGATTGGAAAGCGCGTAATCTAAAAATTTTAAAGAAGTATAATCCAGAAGATGC
ACAAATTGAAGAAATAGATAAAATAATTGATGAATGCCTAGCAAAAATAGAAACATATCTGACTTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11548.17 Da
• Isoelectric Point: 6.4794

>AT23235 WP_012573895.1 NC_011567:176869-177177 [Anoxybacillus flavithermus WK1]
MDSKEHLESNINRNKGGDYMATVVAQKWGNSLGIRIPKDVADKIGIKQGSEMELNVIGNEGIITLKPKRTRKKYTLEELI
SQITPENRHEEIDFGIEGRELI

Nucleotide

• Length: 309 bp

>AT23235 NC_011567:176869-177177 [Anoxybacillus flavithermus WK1]
ATGGATTCAAAGGAACATTTAGAAAGCAACATAAACCGAAATAAAGGGGGAGATTACATGGCTACAGTTGTTGCGCAAAA
ATGGGGAAACAGTTTAGGGATTCGTATCCCAAAGGATGTTGCTGATAAAATAGGGATCAAACAAGGCTCAGAAATGGAAT
TAAATGTGATTGGAAATGAAGGCATTATTACATTAAAGCCAAAAAGAACACGGAAAAAATACACATTGGAAGAACTCATC
TCACAGATTACACCTGAAAATCGACACGAGGAAATAGATTTTGGGATAGAAGGGCGTGAATTAATTTAA

Structures

Toxin

• AlphaFold DB: B7GJ05

Antitoxin

• AlphaFold DB: B7GJ04