Detailed information of TA system TA023234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 1963337..1963866
• Replicon: chromosome
• Accession: NC_011528
• Organism: Coxiella burnetii CbuK Q154

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q83AB3
• Locus tag: CBUK_RS10335
• Protein ID: WP_005769718.1
• Coordinates: 1963591..1963866 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q83AB4
• Locus tag: CBUK_RS10330
• Protein ID: WP_005769716.1
• Coordinates: 1963337..1963591 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBUK_RS10295	1958404..1958778	+	375	WP_005769706.1	hypothetical protein	-
CBUK_RS10300	1958823..1959521	+	699	WP_010958581.1	outer membrane beta-barrel protein	-
CBUK_RS10305	1959585..1960526	+	942	WP_005769709.1	S49 family peptidase	-
CBUK_RS10310	1960539..1961414	+	876	WP_005769711.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-
CBUK_RS12175	1961390..1961503	+	114	Protein_2003	type II toxin-antitoxin system YoeB family toxin	-
CBUK_RS10315	1961563..1961697	+	135	WP_010958583.1	hypothetical protein	-
CBUK_RS10320	1961857..1962877	+	1021	Protein_2005	IS110-like element IS1111A family transposase	-
CBUK_RS10325	1962874..1963146	-	273	WP_005768315.1	hypothetical protein	-
CBUK_RS10330	1963337..1963591	+	255	WP_005769716.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CBUK_RS10335	1963591..1963866	+	276	WP_005769718.1	Txe/YoeB family addiction module toxin	Toxin
CBUK_RS10340	1963839..1964324	-	486	WP_005769725.1	dihydrofolate reductase	-
CBUK_RS10345	1964321..1965124	-	804	WP_012570935.1	epoxyqueuosine reductase QueH	-
CBUK_RS10350	1965320..1965607	+	288	WP_005769730.1	acylphosphatase	-
CBUK_RS10355	1965583..1966137	-	555	WP_005769732.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
CBUK_RS10360	1966150..1967094	-	945	WP_005769734.1	methionyl-tRNA formyltransferase	-
CBUK_RS10365	1967282..1968208	+	927	WP_012570936.1	DNA-processing protein DprA	-
CBUK_RS10370	1968205..1968684	+	480	WP_011997413.1	DUF494 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11150.86 Da
• Isoelectric Point: 9.6762

>T23234 WP_005769718.1 NC_011528:1963591-1963866 [Coxiella burnetii CbuK_Q154]
MQISFTPEAWEDYLYWQKFDKKMLRRINELIKDAMHEPFSGKGKPEPLKFELQGYWSRRLDQEHRLVYKVLDDSLMIIAA
RFHYNRLNSKN

Nucleotide

• Length: 276 bp

>T23234 NC_011528:1963591-1963866 [Coxiella burnetii CbuK_Q154]
ATGCAAATTTCCTTCACGCCCGAAGCCTGGGAAGATTATTTATATTGGCAAAAATTCGACAAAAAAATGCTTCGACGCAT
TAATGAACTCATTAAGGATGCTATGCACGAGCCTTTTTCCGGAAAGGGAAAGCCAGAACCTTTAAAATTTGAATTACAAG
GATATTGGTCAAGACGATTAGATCAAGAACATCGATTGGTCTACAAAGTTTTAGACGATTCGTTAATGATTATCGCCGCA
AGATTTCACTATAATCGCCTTAATTCTAAAAACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9624.25 Da
• Isoelectric Point: 10.4802

>AT23234 WP_005769716.1 NC_011528:1963337-1963591 [Coxiella burnetii CbuK_Q154]
MNVVTFSELRAQLKKILDLSADQHEPVVVKRPNKETMVILSLRDFEALKETAYLLSNEANAARLRQSIRSLKQGKAQKKK
LMED

Nucleotide

• Length: 255 bp

>AT23234 NC_011528:1963337-1963591 [Coxiella burnetii CbuK_Q154]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCTCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Structures

Toxin

• AlphaFold DB: Q83AB3

Antitoxin

• AlphaFold DB: Q83AB4