Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4711573..4712070 Replicon chromosome
Accession NZ_CP091016
Organism Escherichia coli strain STEC306

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L1O96_RS23785 Protein ID WP_001173343.1
Coordinates 4711573..4711746 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag L1O96_RS23790 Protein ID WP_001425728.1
Coordinates 4711774..4712070 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O96_RS23765 (4706907) 4706907..4708364 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
L1O96_RS23770 (4708601) 4708601..4709386 + 786 Protein_4666 OB-fold nucleic acid binding domain-containing protein -
L1O96_RS23780 (4710717) 4710717..4711454 + 738 Protein_4668 lysine--tRNA ligase -
L1O96_RS23785 (4711573) 4711573..4711746 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L1O96_RS23790 (4711774) 4711774..4712070 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L1O96_RS23795 (4712297) 4712297..4712569 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
L1O96_RS23800 (4712581) 4712581..4712811 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
L1O96_RS23805 (4712992) 4712992..4714623 + 1632 WP_001216487.1 sensor histidine kinase -
L1O96_RS23810 (4714620) 4714620..4715339 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4699433..4712070 12637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T232328 WP_001173343.1 NZ_CP091016:c4711746-4711573 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T232328 NZ_CP127276:c1388200-1387907 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT232328 WP_001425728.1 NZ_CP091016:c4712070-4711774 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT232328 NZ_CP127276:c1388466-1388197 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References