Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3884625..3885243 Replicon chromosome
Accession NZ_CP091016
Organism Escherichia coli strain STEC306

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L1O96_RS19790 Protein ID WP_001291435.1
Coordinates 3885025..3885243 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L1O96_RS19785 Protein ID WP_000344800.1
Coordinates 3884625..3884999 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O96_RS19775 (3879714) 3879714..3880907 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L1O96_RS19780 (3880930) 3880930..3884079 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
L1O96_RS19785 (3884625) 3884625..3884999 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L1O96_RS19790 (3885025) 3885025..3885243 + 219 WP_001291435.1 HHA domain-containing protein Toxin
L1O96_RS19795 (3885415) 3885415..3885966 + 552 WP_000102568.1 maltose O-acetyltransferase -
L1O96_RS19800 (3886082) 3886082..3886552 + 471 WP_000136192.1 YlaC family protein -
L1O96_RS19805 (3886716) 3886716..3888266 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L1O96_RS19810 (3888308) 3888308..3888661 - 354 WP_000878140.1 DUF1428 domain-containing protein -
L1O96_RS19820 (3889040) 3889040..3889351 + 312 WP_000409911.1 MGMT family protein -
L1O96_RS19825 (3889382) 3889382..3889954 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T232325 WP_001291435.1 NZ_CP091016:3885025-3885243 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T232325 NZ_CP127276:c1230193-1229882 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT232325 WP_000344800.1 NZ_CP091016:3884625-3884999 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT232325 NZ_CP127276:c1230513-1230193 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References