Detailed information of TA system TA023232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/Txe-RelB
• Location: 1915455..1915984
• Replicon: chromosome
• Accession: NC_011527
• Organism: Coxiella burnetii CbuG Q212

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q83AB3
• Locus tag: CBUG_RS10135
• Protein ID: WP_005769718.1
• Coordinates: 1915709..1915984 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q83AB4
• Locus tag: CBUG_RS10130
• Protein ID: WP_005769716.1
• Coordinates: 1915455..1915709 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBUG_RS10095	1910557..1910931	+	375	WP_005769706.1	hypothetical protein	-
CBUG_RS10100	1910976..1911674	+	699	WP_010958581.1	outer membrane beta-barrel protein	-
CBUG_RS10105	1911738..1912679	+	942	WP_005769709.1	S49 family peptidase	-
CBUG_RS10110	1912692..1913567	+	876	WP_012570511.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-
CBUG_RS11650	1913543..1913656	+	114	Protein_1962	type II toxin-antitoxin system YoeB family toxin	-
CBUG_RS10115	1913716..1913850	+	135	WP_012570512.1	hypothetical protein	-
CBUG_RS10120	1914026..1915045	+	1020	WP_043881149.1	IS110-like element IS1111A family transposase	-
CBUG_RS10125	1915042..1915314	-	273	WP_010957319.1	hypothetical protein	-
CBUG_RS10130	1915455..1915709	+	255	WP_005769716.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CBUG_RS10135	1915709..1915984	+	276	WP_005769718.1	Txe/YoeB family addiction module toxin	Toxin
CBUG_RS10140	1915957..1916442	-	486	WP_005769725.1	dihydrofolate reductase	-
CBUG_RS10145	1916439..1917242	-	804	WP_010958584.1	epoxyqueuosine reductase QueH	-
CBUG_RS10150	1917438..1917725	+	288	WP_032138540.1	acylphosphatase	-
CBUG_RS10155	1917701..1918255	-	555	WP_005769732.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
CBUG_RS10160	1918268..1919212	-	945	WP_010958586.1	methionyl-tRNA formyltransferase	-
CBUG_RS10165	1919400..1920326	+	927	WP_012570515.1	DNA-processing protein DprA	-
CBUG_RS10170	1920323..1920802	+	480	WP_010958588.1	DUF494 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1914026..1915045	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11150.86 Da
• Isoelectric Point: 9.6762

>T23232 WP_005769718.1 NC_011527:1915709-1915984 [Coxiella burnetii CbuG_Q212]
MQISFTPEAWEDYLYWQKFDKKMLRRINELIKDAMHEPFSGKGKPEPLKFELQGYWSRRLDQEHRLVYKVLDDSLMIIAA
RFHYNRLNSKN

Nucleotide

• Length: 276 bp

>T23232 NC_011527:1915709-1915984 [Coxiella burnetii CbuG_Q212]
ATGCAAATTTCCTTCACGCCCGAAGCCTGGGAAGATTATTTATATTGGCAAAAATTCGACAAAAAAATGCTTCGACGCAT
TAATGAACTCATTAAGGATGCTATGCACGAGCCTTTTTCCGGAAAGGGAAAGCCAGAACCTTTAAAATTTGAATTACAAG
GATATTGGTCAAGACGATTAGATCAAGAACATCGATTGGTCTACAAAGTTTTAGACGATTCGTTAATGATTATCGCCGCA
AGATTTCACTATAATCGCCTTAATTCTAAAAACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9624.25 Da
• Isoelectric Point: 10.4802

>AT23232 WP_005769716.1 NC_011527:1915455-1915709 [Coxiella burnetii CbuG_Q212]
MNVVTFSELRAQLKKILDLSADQHEPVVVKRPNKETMVILSLRDFEALKETAYLLSNEANAARLRQSIRSLKQGKAQKKK
LMED

Nucleotide

• Length: 255 bp

>AT23232 NC_011527:1915455-1915709 [Coxiella burnetii CbuG_Q212]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCGCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Structures

Toxin

• AlphaFold DB: Q83AB3

Antitoxin

• AlphaFold DB: Q83AB4