Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 995101..995755 Replicon chromosome
Accession NZ_CP091016
Organism Escherichia coli strain STEC306

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L1O96_RS04895 Protein ID WP_000244777.1
Coordinates 995348..995755 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L1O96_RS04890 Protein ID WP_000354046.1
Coordinates 995101..995367 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O96_RS04865 (990389) 990389..991132 + 744 WP_000951948.1 SDR family oxidoreductase -
L1O96_RS04870 (991189) 991189..992622 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
L1O96_RS04875 (992667) 992667..992978 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
L1O96_RS04880 (993142) 993142..993801 + 660 WP_000250274.1 hemolysin III family protein -
L1O96_RS04885 (993878) 993878..994858 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L1O96_RS04890 (995101) 995101..995367 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L1O96_RS04895 (995348) 995348..995755 + 408 WP_000244777.1 protein YgfX Toxin
L1O96_RS04900 (995795) 995795..996316 - 522 WP_001055874.1 flavodoxin FldB -
L1O96_RS04905 (996428) 996428..997324 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L1O96_RS04910 (997349) 997349..998059 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L1O96_RS04915 (998065) 998065..999798 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T232307 WP_000244777.1 NZ_CP091016:995348-995755 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T232307 NZ_CP127276:362698-363000 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT232307 WP_000354046.1 NZ_CP091016:995101-995367 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT232307 NZ_CP127276:363048-363269 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References