Detailed information of TA system TA023229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3122523..3123155
• Replicon: chromosome
• Accession: NC_011420
• Organism: Rhodospirillum centenum SW (Rhodocista centenaria SW)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RC1_RS20895
• Protein ID: WP_083759333.1
• Coordinates: 3122523..3122930 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B6IVU1
• Locus tag: RC1_RS14570
• Protein ID: WP_012568195.1
• Coordinates: 3122931..3123155 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC1_RS14535	3117637..3118077	+	441	WP_012568187.1	flagellar basal body rod protein FlgC	-
RC1_RS14540	3118150..3119535	+	1386	WP_148213468.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
RC1_RS14545	3119532..3119987	+	456	WP_012568189.1	dUTP diphosphatase	-
RC1_RS14550	3120091..3120348	+	258	WP_012568190.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
RC1_RS14555	3120348..3120752	+	405	WP_012568191.1	type II toxin-antitoxin system VapC family toxin	-
RC1_RS14560	3120931..3121386	+	456	WP_012568192.1	Rrf2 family transcriptional regulator	-
RC1_RS14565	3121459..3122460	+	1002	WP_012568193.1	cysteine synthase A	-
RC1_RS20895	3122523..3122930	-	408	WP_083759333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RC1_RS14570	3122931..3123155	-	225	WP_012568195.1	hypothetical protein	Antitoxin
RC1_RS14575	3123255..3124085	+	831	WP_012568196.1	molybdopterin-synthase adenylyltransferase MoeB	-
RC1_RS14580	3124078..3124503	+	426	WP_012568197.1	hypothetical protein	-
RC1_RS14585	3124580..3125452	+	873	WP_012568198.1	bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase	-
RC1_RS14590	3125467..3125868	+	402	WP_012568199.1	hypothetical protein	-
RC1_RS14595	3125896..3126672	+	777	WP_012568200.1	enoyl-CoA hydratase	-
RC1_RS14600	3126811..3127077	+	267	WP_012568201.1	30S ribosomal protein S20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14853.95 Da
• Isoelectric Point: 5.6885

>T23229 WP_083759333.1 NC_011420:c3122930-3122523 [Rhodospirillum centenum SW]
VLRFMLDTNVCIRVLRDRPQGVRQRFNDEAGSLCISVVTLGELLHGAARSARPAAVREDVERFAERLDVLAFDADAAGHF
ADIRASLERIGQVIGPYDLMIAGHARSRGLTVVTGNLGEFRRVEGLIAEDWLAPE

Nucleotide

• Length: 408 bp

>T23229 NC_011420:c3122930-3122523 [Rhodospirillum centenum SW]
GTGCTTCGTTTCATGTTGGACACGAATGTCTGCATCCGGGTCCTTCGGGACCGGCCGCAGGGCGTGCGCCAACGCTTCAA
CGACGAGGCTGGCAGCCTTTGCATTTCAGTCGTTACCCTGGGCGAGCTTCTGCATGGTGCCGCCCGGTCGGCCCGTCCGG
CAGCCGTCAGAGAGGACGTGGAGCGGTTCGCCGAACGTCTTGATGTTCTTGCCTTCGATGCGGATGCAGCCGGGCATTTC
GCGGATATCCGGGCGTCGCTCGAACGGATTGGCCAGGTTATCGGGCCCTATGACCTCATGATTGCGGGACATGCGCGCAG
CCGAGGGCTTACGGTCGTGACTGGCAATCTCGGCGAATTCCGCCGCGTTGAAGGACTGATCGCGGAAGACTGGCTGGCAC
CCGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8223.47 Da
• Isoelectric Point: 10.7407

>AT23229 WP_012568195.1 NC_011420:c3123155-3122931 [Rhodospirillum centenum SW]
MTVRTKLLRSNSRQAVRLPKEIAFPDGVRDVMILKQGAARLIVPATGAWDDFFDAPGTDLPERAQPAAQKRESF

Nucleotide

• Length: 225 bp

>AT23229 NC_011420:c3123155-3122931 [Rhodospirillum centenum SW]
ATGACCGTTCGCACGAAACTGCTCCGGTCGAACAGCCGGCAGGCGGTCCGGCTGCCGAAGGAGATCGCCTTTCCCGACGG
TGTGCGGGACGTGATGATCCTGAAGCAGGGTGCCGCCCGGCTGATCGTCCCGGCAACCGGAGCCTGGGACGATTTCTTCG
ATGCCCCGGGCACCGACCTGCCGGAGCGCGCGCAACCTGCCGCACAGAAGCGGGAGAGCTTCTGA

Structures

Antitoxin

• AlphaFold DB: B6IVU1