Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1646080..1646609 Replicon chromosome
Accession NZ_CP090985
Organism Staphylococcus epidermidis strain 50D

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag KB226_RS08015 Protein ID WP_001829891.1
Coordinates 1646080..1646442 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag KB226_RS08020 Protein ID WP_001829931.1
Coordinates 1646439..1646609 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KB226_RS07995 (KB226_07995) 1643082..1643852 - 771 WP_002440602.1 RNA polymerase sigma factor SigB -
KB226_RS08000 (KB226_08000) 1643827..1644306 - 480 WP_001829903.1 anti-sigma B factor RsbW -
KB226_RS08005 (KB226_08005) 1644308..1644634 - 327 WP_001829952.1 anti-sigma factor antagonist -
KB226_RS08010 (KB226_08010) 1644734..1645735 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
KB226_RS08015 (KB226_08015) 1646080..1646442 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KB226_RS08020 (KB226_08020) 1646439..1646609 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KB226_RS08025 (KB226_08025) 1646696..1647844 - 1149 WP_002457107.1 alanine racemase -
KB226_RS08030 (KB226_08030) 1647911..1648264 - 354 WP_029376481.1 holo-ACP synthase -
KB226_RS08035 (KB226_08035) 1648312..1648821 - 510 WP_235075570.1 PH domain-containing protein -
KB226_RS08040 (KB226_08040) 1648808..1650313 - 1506 WP_029376482.1 PH domain-containing protein -
KB226_RS08045 (KB226_08045) 1650306..1650785 - 480 WP_002457111.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T232271 WP_001829891.1 NZ_CP090985:c1646442-1646080 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T232271 NZ_CP127275:c1570783-1570382 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT232271 WP_001829931.1 NZ_CP090985:c1646609-1646439 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT232271 NZ_CP127275:c1571037-1570780 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References