Detailed information of TA system TA023227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 3001293..3001905
• Replicon: chromosome
• Accession: NC_011420
• Organism: Rhodospirillum centenum SW (Rhodocista centenaria SW)

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B6IVG6
• Locus tag: RC1_RS13980
• Protein ID: WP_012568070.1
• Coordinates: 3001636..3001905 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: B6IVG5
• Locus tag: RC1_RS13975
• Protein ID: WP_012568069.1
• Coordinates: 3001293..3001616 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC1_RS13955	2996541..2996744	+	204	WP_041785386.1	hypothetical protein	-
RC1_RS13960	2996893..2997366	+	474	WP_012568066.1	hypothetical protein	-
RC1_RS13965	2997486..2999609	+	2124	WP_012568067.1	polyribonucleotide nucleotidyltransferase	-
RC1_RS13970	2999725..3001092	+	1368	WP_041785389.1	IS1182-like element ISRce2 family transposase	-
RC1_RS13975	3001293..3001616	+	324	WP_012568069.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
RC1_RS13980	3001636..3001905	+	270	WP_012568070.1	Txe/YoeB family addiction module toxin	Toxin
RC1_RS13985	3002346..3003005	+	660	WP_041785392.1	porin family protein	-
RC1_RS13990	3003274..3003924	+	651	WP_184446351.1	outer membrane beta-barrel protein	-
RC1_RS13995	3003992..3004570	-	579	WP_012568073.1	DUF882 domain-containing protein	-
RC1_RS14000	3004682..3005554	-	873	WP_012568074.1	MBL fold metallo-hydrolase	-
RC1_RS14005	3005623..3006309	-	687	WP_012568075.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2999695..3001092	1397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (25-97)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10331.80 Da
• Isoelectric Point: 10.2449

>T23227 WP_012568070.1 NC_011420:3001636-3001905 [Rhodospirillum centenum SW]
MRISFSSGAWEDYLHWQEQDRAILARVNTLIRECTRAPFVGLGKPEPLRGSLAGWWSRRITGEHRLVYRVAGQPPDQALE
IAACRRHYR

Nucleotide

• Length: 270 bp

>T23227 NC_011420:3001636-3001905 [Rhodospirillum centenum SW]
ATGCGCATCAGTTTCAGCAGCGGGGCCTGGGAGGATTATCTGCACTGGCAGGAGCAGGACCGGGCCATCCTGGCACGGGT
GAACACGCTGATCCGGGAGTGTACGCGCGCACCCTTCGTCGGCCTGGGCAAGCCCGAACCGCTGCGCGGTTCCCTGGCGG
GCTGGTGGTCACGGCGTATCACCGGGGAACACCGGCTCGTCTATCGCGTGGCCGGACAGCCGCCCGATCAGGCGCTGGAG
ATCGCGGCCTGCCGGCGGCATTACCGATAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11728.96 Da
• Isoelectric Point: 4.6346

>AT23227 WP_012568069.1 NC_011420:3001293-3001616 [Rhodospirillum centenum SW]
MPDAQRLHDDDDDGGRADPRSVGARHVSATEFRQNLARHLDQVRDSRAPLVVTRQGEEGVVVLAEGEWAALQETLHVLGT
PANARRLLDAIAEFEAGKGVERDLIEP

Nucleotide

• Length: 324 bp

>AT23227 NC_011420:3001293-3001616 [Rhodospirillum centenum SW]
ATGCCCGATGCCCAGCGGCTCCACGACGACGACGACGATGGCGGCCGCGCTGACCCCCGCAGCGTCGGCGCGCGCCATGT
CAGCGCCACGGAGTTCCGGCAGAATCTGGCCCGCCATCTCGATCAGGTCCGCGACAGCCGTGCGCCTCTTGTGGTGACCC
GCCAGGGGGAAGAGGGCGTCGTCGTTCTGGCGGAGGGCGAATGGGCGGCCCTCCAGGAAACCCTCCACGTCCTGGGCACA
CCGGCCAACGCGCGGCGGCTCCTGGACGCCATCGCCGAGTTCGAGGCGGGTAAGGGTGTCGAGCGCGACCTGATCGAGCC
CTGA

Structures

Toxin

• AlphaFold DB: B6IVG6

Antitoxin

• AlphaFold DB: B6IVG5