Detailed information of TA system TA232266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 754808..755413
• Replicon: chromosome
• Accession: NZ_CP090978
• Organism: Paenibacillus sp. YPD9-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A1V4HIK6
• Locus tag: L0M14_RS03385
• Protein ID: WP_028553893.1
• Coordinates: 755063..755413 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: L0M14_RS03380
• Protein ID: WP_235122797.1
• Coordinates: 754808..755059 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0M14_RS03360 (L0M14_03360)	749844..751142	+	1299	WP_235120827.1	outer membrane lipoprotein-sorting protein	-
L0M14_RS03365 (L0M14_03365)	752657..752749	+	93	Protein_659	SEC-C metal-binding domain-containing protein	-
L0M14_RS03370 (L0M14_03370)	752932..753276	+	345	Protein_660	alanine racemase	-
L0M14_RS03375 (L0M14_03375)	753712..754527	+	816	Protein_661	alanine racemase	-
L0M14_RS03380 (L0M14_03380)	754808..755059	+	252	WP_235122797.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0M14_RS03385 (L0M14_03385)	755063..755413	+	351	WP_028553893.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L0M14_RS03390 (L0M14_03390)	755655..757807	+	2153	Protein_664	RNA-binding transcriptional accessory protein	-
L0M14_RS03395 (L0M14_03395)	758422..759057	-	636	WP_235120830.1	hypothetical protein	-
L0M14_RS03400 (L0M14_03400)	759249..759413	-	165	WP_235120831.1	cortex morphogenetic protein CmpA	-
L0M14_RS03405 (L0M14_03405)	759549..759908	-	360	WP_235120833.1	hydrolase/acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12892.81 Da
• Isoelectric Point: 4.8837

>T232266 WP_028553893.1 NZ_CP090978:755063-755413 [Paenibacillus sp. YPD9-1]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAETHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMRKVDDALQISVGLIEF

Nucleotide

• Length: 351 bp

>T232266 NZ_CP127275:c1227284-1226973 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9541.88 Da
• Isoelectric Point: 4.6561

>AT232266 WP_235122797.1 NZ_CP090978:754808-755059 [Paenibacillus sp. YPD9-1]
MISLPDNLLQEVDGIVEKENSNRSEFIRQAMKLYLIERKKRSIRESMQRGYMEMAKINLSMASEAFQAEEDADSTVDRLV
SGV

Nucleotide

• Length: 252 bp

>AT232266 NZ_CP127275:c1227604-1227284 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Structures

Toxin

• AlphaFold DB: A0A1V4HIK6