Detailed information of TA system TA023225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1981..2540
• Replicon: plasmid pSE11-1
• Accession: NC_011419
• Organism: Escherichia coli SE11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E0J3F0
• Locus tag: ECSE_RS24440
• Protein ID: WP_000421267.1
• Coordinates: 2265..2540 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: ECSE_RS24435
• Protein ID: WP_001178087.1
• Coordinates: 1981..2265 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSE_RS24430	46..1077	+	1032	WP_000907860.1	replication initiation protein	-
ECSE_RS24435	1981..2265	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
ECSE_RS24440	2265..2540	+	276	WP_000421267.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECSE_RS24445	2646..2933	+	288	WP_001105073.1	hypothetical protein	-
ECSE_RS28960	3124..3390	-	267	WP_000627834.1	hypothetical protein	-
ECSE_RS24455	3390..3884	-	495	WP_001350009.1	hypothetical protein	-
ECSE_RS24460	3940..4281	-	342	WP_000411278.1	hypothetical protein	-
ECSE_RS24465	4357..5061	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
ECSE_RS28815	5108..5257	-	150	WP_158299169.1	hypothetical protein	-
ECSE_RS27835	5977..6516	-	540	Protein_9	helix-turn-helix transcriptional regulator	-
ECSE_RS24480	6503..7162	-	660	WP_001038045.1	tetracycline resistance transcriptional repressor TetR(C)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(C)	-	1..100021	100021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10788.48 Da
• Isoelectric Point: 9.9325

>T23225 WP_000421267.1 NC_011419:2265-2540 [Escherichia coli SE11]
MELKWTSKALSDLARLYDFLVLTSKPAAARTVQSLTHAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T23225 NC_011419:2265-2540 [Escherichia coli SE11]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGACCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACATGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT23225 WP_001178087.1 NC_011419:1981-2265 [Escherichia coli SE11]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT23225 NC_011419:1981-2265 [Escherichia coli SE11]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: U9Y625