Detailed information of TA system TA232249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3237185..3238905
• Replicon: chromosome
• Accession: NZ_CP090967
• Organism: Edwardsiella piscicida strain KE5

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: D0ZER0
• Locus tag: L1P07_RS14725
• Protein ID: WP_012849887.1
• Coordinates: 3237185..3237841 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: L1P07_RS14730
• Protein ID: WP_226989538.1
• Coordinates: 3237877..3238905 (+)
• Length: 343 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P07_RS14705 (L1P07_14705)	3232549..3232788	+	240	WP_012849883.1	hypothetical protein	-
L1P07_RS14710 (L1P07_14710)	3232827..3235265	+	2439	WP_012849884.1	type I restriction-modification system subunit M	-
L1P07_RS14715 (L1P07_14715)	3235259..3236602	+	1344	WP_226989537.1	restriction endonuclease subunit S	-
L1P07_RS14720 (L1P07_14720)	3236607..3237182	+	576	WP_012849886.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
L1P07_RS14725 (L1P07_14725)	3237185..3237841	+	657	WP_012849887.1	DUF4433 domain-containing protein	Toxin
L1P07_RS14730 (L1P07_14730)	3237877..3238905	+	1029	WP_226989538.1	macro domain-containing protein	Antitoxin
L1P07_RS14735 (L1P07_14735)	3239069..3242164	+	3096	WP_012849889.1	HsdR family type I site-specific deoxyribonuclease	-
L1P07_RS14740 (L1P07_14740)	3242164..3242880	+	717	WP_012849890.1	SprT family zinc-dependent metalloprotease	-
L1P07_RS14745 (L1P07_14745)	3243125..3243664	+	540	WP_012849891.1	transposase DNA-binding-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3214894..3260374	45480
	-	flank	IS/Tn	-	-	3243678..3244559	881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (5-116)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25146.59 Da
• Isoelectric Point: 6.7624

>T232249 WP_012849887.1 NZ_CP090967:3237185-3237841 [Edwardsiella piscicida]
VAYDYSANLNPEKALIWRIVHRDNIPWILDHGLHCGNSPVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRNVAAQNIPFVFTDSHAYYNWVNYYTRLDDLNQIDWSILQARDFRRDPDD
PAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T232249 NZ_CP127275:360669-361094 [Mycobacterium tuberculosis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 343 a.a.
• Molecular weight: 39294.39 Da
• Isoelectric Point: 7.1506

>AT232249 WP_226989538.1 NZ_CP090967:3237877-3238905 [Edwardsiella piscicida]
VEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTKQHWRADSRMAWIE
DGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESTLGDLQDTEILIYEPTEKYQNVAKSSGVKKLTPARAMIA
ELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDV
IWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEGLHQWPAGEPWASR
KIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1029 bp

>AT232249 NZ_CP127275:360451-360672 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Toxin

• AlphaFold DB: D0ZER0